MONOPTEROS directly activates the auxin-inducible promoter of the Dof5.8 transcription factor gene in Arabidopsis thaliana leaf provascular cells
| Autor: | Mineko Konishi, Shuichi Yanagisawa, Enrico Scarpella, Tyler J. Donner |
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| Rok vydání: | 2014 |
| Předmět: |
0106 biological sciences
auxin response Arabidopsis thaliana Physiology Mutant Arabidopsis Plant Science 01 natural sciences 03 medical and health sciences Gene Expression Regulation Plant Auxin Primordium vascular development Promoter Regions Genetic MONOPTEROS Gene Transcription factor 030304 developmental biology chemistry.chemical_classification Genetics 0303 health sciences Indoleacetic Acids biology Arabidopsis Proteins food and beverages embryo development biology.organism_classification Phenotype Cell biology DNA-Binding Proteins Plant Leaves Repressor Proteins chemistry Dof transcription factor Transcription Factor Gene Research Paper Transcription Factors 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/eru418 |
| Popis: | Highlight An Arabidopsis gene, Dof5.8, is a target of the auxin-responsive MONOPTEROS transcription factor. Mutations in Dof5.8 interfere with embryo development and vein patterning when combined with a monopteros allele. MONOPTEROS (MP) is an auxin-responsive transcription factor that is required for primary root formation and vascular development, whereas Dof5.8 is a Dof-class transcription factor whose gene is expressed in embryos as well as the pre- and procambial cells in the leaf primordium in Arabidopsis thaliana. In this study, it is shown that MP directly activates the Dof5.8 promoter. Although no apparent phenotype of the single dof5.8 mutants was found, phenotypic analysis with the mp dof5.8 double mutants revealed that mutations within Dof5.8 enhanced the phenotype of a weak allele of mp, with an increase in the penetrance of the ‘rootless’ phenotype and a reduction in the number of cotyledons. Furthermore, interestingly, although mp mutants showed reduced vascular pattern complexity in cotyledons, the mp dof5.8 double mutants displayed both more simplex and more complex vascular patterns in individual cotyledons. These results imply that the product of Dof5.8 whose expression is regulated by MP at least in part might be involved in multiple processes controlled by MP. |
| Databáze: | OpenAIRE |
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