The tripartite motif coiled-coil is an elongated antiparallel hairpin dimer
| Autor: | Jacint G. Sanchez, Viswanathan Chandrasekaran, Wesley I. Sundquist, Jordan M. Welker, Katarzyna Okreglicka, Owen Pornillos |
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| Rok vydání: | 2014 |
| Předmět: |
Models
Molecular TRIM25 Protein Conformation Stereochemistry Ubiquitin-Protein Ligases viruses Molecular Sequence Data Biology Crystallography X-Ray Antiparallel (biochemistry) Trim Evolution Molecular Tripartite Motif Proteins Protein structure Rosaniline Dyes Humans Fluorometry Coiled coil Multidisciplinary Base Sequence Sequence Analysis DNA Biological Sciences Tripartite motif family Protein Structure Tertiary Crystallography Dimerization Ultracentrifugation TRIM Family Transcription Factors |
| Zdroj: | Proceedings of the National Academy of Sciences. 111:2494-2499 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1318962111 |
| Popis: | Tripartite motif (TRIM) proteins make up a large family of coiled-coil-containing RING E3 ligases that function in many cellular processes, particularly innate antiviral response pathways. Both dimerization and higher-order assembly are important elements of TRIM protein function, but the atomic details of TRIM tertiary and quaternary structure have not been fully understood. Here, we present crystallographic and biochemical analyses of the TRIM coiled-coil and show that TRIM proteins dimerize by forming interdigitating antiparallel helical hairpins that position the N-terminal catalytic RING domains at opposite ends of the dimer and the C-terminal substrate-binding domains at the center. The dimer core comprises an antiparallel coiled-coil with a distinctive, symmetric pattern of flanking heptad and central hendecad repeats that appear to be conserved across the entire TRIM family. Our studies reveal how the coiled-coil organizes TRIM25 to polyubiquitylate the RIG-I/viral RNA recognition complex and how dimers of the TRIM5α protein are arranged within hexagonal arrays that recognize the HIV-1 capsid lattice and restrict retroviral replication. |
| Databáze: | OpenAIRE |
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