Cloning and expression of a cDNA encoding a catalytically active fragment of calf thymus DNA polymerase alpha
| Autor: | Kimberly A. Foster, Knut Eliassen, Ulrich Hübscher, Gary K. McMaster, Naseema N. Khan, Kersten Lüthi-Steinmann, Elena Ferrari, Marjorie H. Barnes, Neal C. Brown |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Cloning
Base Sequence biology DNA polymerase Recombinant Fusion Proteins Structural gene Biophysics DNA DNA Polymerase II Cell Biology beta-Galactosidase Biochemistry Fusion protein Molecular biology Chromatography Affinity Insert (molecular biology) Complementary DNA biology.protein RNA polymerase I Cloning Molecular Molecular Biology Polymerase |
| Zdroj: | Biochemical and Biophysical Research Communications. 140:21-27 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(86)91052-1 |
| Popis: | A calf thymus cDNA expression library was constructed in the EcoRI site of lambda gt11 and probed with an antibody raised against calf thymus DNA polymerase alpha. Three classes of antibody-reactive clones were isolated. The largest class carried a 1.9 kilobase calf cDNA insert and expressed a 165-175 kilodalton beta-galactosidase:calf fusion protein which displayed DNA polymerase activity. The characteristic responses of the polymerase activity to alpha-specific inhibitors and antibodies identified the 1.9 kilobase cDNA as a sequence specifically derived from the structural gene encoding the pol alpha catalytic core. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|