Oligonucleotide primers for specific detection of actinobacterial laccases from superfamilies I and K
| Autor: | Tiago Domingues Zucchi, Wendel Batista da Silveira, Tatiana Alves Rigamonte Fernandes, Flávia M. L. Passos |
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| Rok vydání: | 2014 |
| Předmět: |
DNA
Bacterial Specific detection Molecular Sequence Data Computational biology Biology Multicopper oxidase DNA Ribosomal Polymerase Chain Reaction Microbiology RNA Ribosomal 16S Cluster Analysis Molecular Biology Gene Phylogeny DNA Primers chemistry.chemical_classification Laccase Phylogenetic tree Phylum Sequence Analysis DNA General Medicine Oligonucleotide primers Actinobacteria Enzyme chemistry Sequence Alignment |
| Zdroj: | Antonie van Leeuwenhoek. 106:391-398 |
| ISSN: | 1572-9699 0003-6072 |
| Popis: | Although many putative laccase-like genes have been assigned to members of the phylum Actinobacteria, few of the related enzymes have been characterized so far. It is noteworthy, however, that this small number of enzymes has presented properties with industrial relevance. This observation, combined with the recognized biotechnological potential and the capability of this phylum to degrade recalcitrant soil polymers, has attracted attention for bioprospective approaches. In the present work, we have designed and tested primers that were specific for detection of sub-groups of laccase-like genes within actinomycetes, which corresponded to the superfamilies I and K from the classification presented by the laccase and multicopper oxidase engineering database. The designed primers have amplified laccase-like gene fragments from actinomycete isolates that were undetectable by primers available from the literature. Furthermore, phylogenetic alignments suggest that some of these fragments may belong to new laccases-like proteins, and thus emphasize the benefits of designing subgroup-specific primers. |
| Databáze: | OpenAIRE |
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