Production of Monoclonal Antibody Against EP0 Protein of Pseudorabies Virus and Determination of Its Recognized Epitope
| Autor: | Yi Cheng, Yikuan Ji, Yongwen Luo, Keyu Xiang, Leilei Sun, Meizhen Zhou, Baoshi Zhang, Yu Wang, Chunmei Ju |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
medicine.diagnostic_test
medicine.drug_class Blotting Western Immunology Antibodies Monoclonal Enzyme-Linked Immunosorbent Assay Original Articles Biology Monoclonal antibody Herpesvirus 1 Suid Virology Molecular biology Epitope Virus Blot Viral Proteins Epitope mapping Western blot Antigen medicine biology.protein Immunology and Allergy Antibody Epitope Mapping |
| Zdroj: | Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 33:409-413 |
| ISSN: | 2167-9436 |
| DOI: | 10.1089/mab.2014.0046 |
| Popis: | Early protein 0 (EP0) is especially important for modulating PRV gene expression and reactivation from the latent state, but the mechanisms have not been elucidated. In this study, six monoclonal antibodies (MAbs) against EP0 protein of PRV were generated and their characterizations were investigated. Western blot analysis showed all six MAbs could react with immunizing antigen, but only 2B12 and 2C6 could react with native EP0 protein from PRV-infected cells. ELISA additivity tests revealed that at least three epitopes in EP0 were defined by six MAbs. The epitope recognized by MAb 2B12 was further identified in 287–292 aa of EP0 protein using a series of expressed overlapping peptides. These MAbs may provide valuable tools for further research of the functions of EP0 in PRV infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|