Optimized Diagnostic Assays Based on Redox Tagged Bioreceptive Interfaces

Autor:	Paulo Roberto Bueno, Flávio C. Bedatty Fernandes, Amol V. Patil, Jason J. Davis
Rok vydání:	2015
Předmět:	Chemistry Interface (computing) Static Electricity Nanotechnology Electrochemical Techniques Transduction (psychology) Assay sensitivity Analytical Chemistry Reduction (complexity) Data acquisition Immittance Static electricity Electrodes Oxidation-Reduction Electrical impedance Biomarkers
Zdroj:	Analytical Chemistry. 87:12137-12144
ISSN:	1520-6882 0003-2700
DOI:	10.1021/acs.analchem.5b02976
Popis:	Among the numerous label free electronic biomarker assay methodologies now available, impedance based electrochemical capacitance spectroscopy (ECS), based upon mapping the perturbations in interfacial charging of redox elements incorporated into a biologically receptive interface, has recently been shown to be a convenient and highly sensitive mode of transduction and one which, additionally, requires no predoping of analytical solution. We present, herein, a data acquisition and analysis methodology based on frequency resolved immittance function analysis. Ultimately, this enables both a maximization of assay sensitivity and a reduction in assay acquisition time by an order of magnitude.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::adc0749c66d7454b233846646f042948 https://doi.org/10.1021/acs.analchem.5b02976 Zobrazit plný text záznamu