Expression of human IL-1alpha after intramarrow gene transfer into healthy non-human primate by adenoviral vector
| Autor: | Tania Sorg, Dominique Dormont, Thierry de Revel, Nicolas Becard, Roger Le Grand |
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| Rok vydání: | 2005 |
| Předmět: |
Male
medicine.medical_treatment Transgene Genetic Vectors Gene Expression Bone Marrow Cells Enzyme-Linked Immunosorbent Assay Biology Viral vector Adenoviridae medicine Animals Humans Platelet Transgenes DNA Primers General Veterinary Monocyte Gene Transfer Techniques Genetic Therapy Hematopoietic Stem Cells Haematopoiesis Macaca fascicularis Cytokine medicine.anatomical_structure C-Reactive Protein Immunology Mauritius Animal Science and Zoology Myelopoiesis Bone marrow Nucleic Acid Amplification Techniques Interleukin-1 |
| Zdroj: | Journal of medical primatology. 34(1) |
| ISSN: | 0047-2565 |
| Popis: | Interleukin-1alpha (IL-1alpha) is a multifunctional cytokine that stimulates myelopoiesis in macaque. However, daily systemic injections of IL-1alpha are associated with severe side effects. We therefore investigated the feasibility of a gene therapy strategy aimed at increasing the IL-1alpha local production in bone marrow with limited release of the vector into the blood circulation. Intra-medullar administration of adenoviral vector containing human IL-1alpha (huIL-1alpha) gene resulted in enhanced neutrophil, monocyte and platelet counts during the two first weeks after injection. The DNA vector, the transgene expression and the huIL-1alpha production was detected in treated bone marrow without significant detection of huIL-1alpha in the peripheral blood. Associated with huIL-1alpha production, we observed concomitant plasma C reactive protein and IL-1Ra peaks in the acellular fraction of treated bone marrow at days 3 and 7. No abnormal clinical side effects were observed in any of the animals following the adenoviral vector injection. |
| Databáze: | OpenAIRE |
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