Spiral ganglion cell morphology in guinea pigs after deafening and neurotrophic treatment
| Autor: | J. C. M. J. De Groot, Martijn J.H. Agterberg, Dyan Ramekers, M.C. van Loon, Sjaak F.L. Klis, Huib Versnel, Wilko Grolman |
|---|---|
| Přispěvatelé: | Otolaryngology / Head & Neck Surgery, EMGO - Quality of care |
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Time Factors Cell Survival DCN MP - Plasticity and memory Guinea Pigs Biophysics Tropomyosin receptor kinase B Deafness Cell morphology Basal (phylogenetics) Furosemide Kanamycin Neurotrophic factors Ganglia Spinal Internal medicine Hair Cells Auditory otorhinolaryngologic diseases medicine Glial cell line-derived neurotrophic factor Animals Cell Shape Spiral ganglion Cochlea Cell Size Neurons biology Brain-Derived Neurotrophic Factor Auditory Threshold Sensory Systems Disease Models Animal medicine.anatomical_structure Endocrinology Nerve Degeneration biology.protein Female sense organs Neuroscience Neurotrophin |
| Zdroj: | Hearing Research, 298, 17-26 van Loon, M C, Ramekers, D, Agterberg, M J H, de Groot, J C M J, Grolman, W, Klis, S F L & Versnel, H 2013, ' Spiral ganglion cell morphology in guinea pigs after deafening and neurotrophic treatment ', Hearing Research, vol. 298, pp. 17-26 . https://doi.org/10.1016/j.heares.2013.01.013 Hearing Research, 298, 17-26. Elsevier Hearing Research, 298, pp. 17-26 |
| ISSN: | 0378-5955 |
| DOI: | 10.1016/j.heares.2013.01.013 |
| Popis: | It is well known that spiral ganglion cells (SGCs) degenerate in hair-cell-depleted cochleas and that treatment with exogenous neurotrophins can prevent this degeneration. Several studies reported that, in addition, SGC size decreases after deafening and increases after neurotrophic treatment. The dynamics of these cell size changes are not well known. In a first experiment we measured size, shape (circularity) and intracellular density of SGCs in guinea pigs at various moments after deafening (1, 2, 4, 6, and 8 weeks) and at various cochlear locations. In a second experiment, the effect of treatment with brain-derived neurotrophic factor (BDNF) on SGC morphology was investigated at various cochlear locations in deafened guinea pigs. We found that SGC size gradually decreased after deafening in the basal and middle cochlear turns. Already after one week a decrease in size was observed, which was well before the number of SGCs started to decrease. After BDNF treatment SGCs became noticeably larger than normal throughout the cochlea, including the middle and apical turns, whereas an effect on survival of SGCs was primarily observed in the basal turn. Thus, both after deafening and after neurotrophic treatment a change in size occurs before survival is affected. Morphological changes were not restricted to a subpopulation of SGCs. We argue that although changes in cell size and changes in survival might be manifestations of two separate mechanisms, morphological measures such as size, circularity and intracellular density are indicative for survival and degeneration. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect