Application of FLiP Method for Differentiation of Mycobacterium tuberculosis Strains in Comparison to Commonly Used Methods, Spoligotyping and MIRU-VNTR Typing
| Autor: | Anna Brzostek, Alina E. Gorna, Anna Zaczek, Anna Sajduda, Arkadiusz Wojtasik, Jarosław Dziadek |
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| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Tuberculosis biology Miru vntr typing Genetic Variation Mycobacterium tuberculosis General Medicine Computational biology bacterial infections and mycoses biology.organism_classification medicine.disease Applied Microbiology and Biotechnology Microbiology Is6110 rflp Discriminatory power Flip medicine bacteria Typing Typing methods Nucleic Acid Amplification Techniques Phylogeny |
| Zdroj: | Polish Journal of Microbiology. 62:73-76 |
| ISSN: | 2544-4646 1733-1331 |
| DOI: | 10.33073/pjm-2013-009 |
| Popis: | The current "gold standard" in molecular epidemiological studies of Mycobacterium tuberculosis is IS6110 RFLP based on IS6110 polymorphism. However PCR-based methods are becoming increasingly important. Recently, fast ligation-mediated PCR (FLiP), based on IS6110 polymorphism was proposed. In this study, the discriminatory power of FLIP, spoligotyping and MIRU-VNTR typing, in differentiation of M. tuberculosis isolates was compared. The discriminatory index (HGI) of spoligotyping, MIRU-VNTR analysis, and FLiP was 0.653, 0.837, and 0.917, respectively. This indicates that FLiP allows a high level of differentiation among M. tuberculosis strains and it might be a valuable alternative to the other typing methods. |
| Databáze: | OpenAIRE |
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