European Sero-Epidemiology Network: standardisation of the assay results for pertussis
| Autor: | A Giammanco, A Chiarini, P.A.C Maple, N Andrews, R Pebody, N Gay, R.M Ölander, F Fivet-Groyne, S Baron, A Tischer, S Swidsinski, J Schellekens, E Reizenstein |
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| Rok vydání: | 2003 |
| Předmět: |
Male
medicine.medical_specialty Adolescent Whooping Cough Enzyme-Linked Immunosorbent Assay Herd immunity medicine Humans Sero epidemiology Medical physics Child High titre Reference standards Whooping cough General Veterinary General Immunology and Microbiology business.industry Public Health Environmental and Occupational Health Reproducibility of Results Reference Standards medicine.disease Antibodies Bacterial Europe Infectious Diseases Italy Child Preschool Calibration Immunology Molecular Medicine Female Tos ferina business |
| Zdroj: | Vaccine. 22:112-120 |
| ISSN: | 0264-410X |
| Popis: | A standardisation process was developed in order to compare and harmonize serological results of pertussis toxin (PT) antibody measurements performed by laboratories using different technical procedures for detection. This involved the development of a common panel, of sera by a designed reference centre, the distribution of the panel to each participating laboratory for testing with their routine methods, the comparison of the obtained results to those of the reference centre, and the calculation of standardisation equations by regressing the quantitative results against those of the reference centre. As a cut-off indicative of protection against pertussis has not yet been defined, a particular emphasis was laid upon achieving standardisation of high titre results that would allow epidemiological evaluations based on the estimation of the incidence of recent infections rather than on the traditional approach of determining the population immunity profile. A generally good agreement was achieved between the participating laboratories, all using ELISA procedures very similar in many crucial aspects, and standardisation equations were produced useful to enable inter-country comparison during the next stages of the European Sero-Epidemiology Network (ESEN) project concerning the serological surveillance of immunity to pertussis in Europe. |
| Databáze: | OpenAIRE |
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