Dapdiamides, Tripeptide Antibiotics Formed by Unconventional Amide Ligases†
| Autor: | Xiaorong Zhang, Jon Clardy, Jessica Dawlaty, Michael A. Fischbach |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Transposable element
Nuclear Magnetic Resonance Medicinal & Biomolecular Chemistry Pharmaceutical Science Erwinia 010402 general chemistry Medical and Health Sciences 01 natural sciences Article Analytical Chemistry Ligases 03 medical and health sciences Drug Discovery Gene cluster Genetics Erwinia amylovora Nuclear Magnetic Resonance Biomolecular 030304 developmental biology Pharmacology 0303 health sciences biology Molecular Structure Pantoea Organic Chemistry Biological Sciences biology.organism_classification Pantoea agglomerans 0104 chemical sciences Anti-Bacterial Agents genomic DNA Infectious Diseases Complementary and alternative medicine Biochemistry Multigene Family Chemical Sciences Cosmid HIV/AIDS Molecular Medicine Transposon mutagenesis Oligopeptides Biomolecular |
| Zdroj: | Journal of Natural Products Journal of natural products, vol 73, iss 3 |
| ISSN: | 1520-6025 0163-3864 |
| Popis: | Construction of a genomic DNA library from Pantoea agglomerans strain CU0119 and screening against the plant pathogen Erwinia amylovora yielded a new family of antibiotics, dapdiamides A-E (1-5). The structures were established through 2D-NMR experiments and mass spectrometry, as well as the synthesis of dapdiamide A (1). Transposon mutagenesis of the active cosmid allowed identification of the biosynthetic gene cluster. The dapdiamide family's promiscuous biosynthetic pathway contains two unconventional amide ligases that are predicted to couple its constituent monomers. |
| Databáze: | OpenAIRE |
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