Revealing the Microscopic Structure of Human Renal Cell Carcinoma in Three Dimensions
| Autor: | Mark Müller, Enken Drecoll, Franz Pfeiffer, Ernst J. Rummeny, Daniela Pfeiffer, Melanie A. Kimm, Madleen Busse, T. Burkner, Simone Ferstl, Sebastian Allner, Martin Dierolf, Wilko Weichert |
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| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Computer science Computed tomography Stain 030218 nuclear medicine & medical imaging 03 medical and health sciences Imaging Three-Dimensional 0302 clinical medicine Renal cell carcinoma Microscopy medicine Humans Electrical and Electronic Engineering Carcinoma Renal Cell Radiological and Ultrasound Technology medicine.diagnostic_test Histological Techniques Soft tissue Histology X-Ray Microtomography Gold standard (test) Tissue morphology medicine.disease Kidney Neoplasms Computer Science Applications Staining Histopathology Software |
| Zdroj: | IEEE Transactions on Medical Imaging. 39:1494-1500 |
| ISSN: | 1558-254X 0278-0062 |
| DOI: | 10.1109/tmi.2019.2952028 |
| Popis: | For fully characterizing renal cell carcinoma (RCC), information about the 3D tissue microstructure is essential. Histopathology, which represents the current diagnostic gold standard, is destructive and only provides 2D information. 3D X-ray histology endeavors to overcome these limitations by generating 3D data. In a laboratory environment, most techniques struggle with limited resolution and the weak X-ray attenuation contrast of soft tissue. We recently developed a laboratory-based method combining nanoscopic X-ray CT with a cytoplasm-specific X-ray stain. Here, we present the application of this method to human RCC biopsies. The NanoCT slices enable pathological characterization of crucial structures by reproducing tissue morphology with a similar detail level as corresponding histological light microscopy images. Beyond that, our data offer deeper insights into the 3D configuration of the tumor. By demonstrating the compatibility of the X-ray stain with standard pathological stains, we highlight the feasibility of integrating staining based NanoCT into the pathological routine. |
| Databáze: | OpenAIRE |
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