Characterization of two myostatin genes in pufferfish Takifugu bimaculatus: sequence, genomic structure, and expression
| Autor: | Keying Liang, Leibin Li, Bo Liu, Haifu Wan, Jianxing Zhong, Xin Zhang, Yinzhen Sheng, Chengjie Yao, Yilei Wang, Yulong Sun, Ziping Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Nervous system
lcsh:Medicine Myostatin mstn1 mstn2 General Biochemistry Genetics and Molecular Biology Muscle hypertrophy 03 medical and health sciences Sequence medicine Myocyte Expression pattern Gene 030304 developmental biology 0303 health sciences biology General Neuroscience Embryogenesis lcsh:R Skeletal muscle 04 agricultural and veterinary sciences General Medicine Cell biology Gastrulation medicine.anatomical_structure Takifugu bimaculatus 040102 fisheries biology.protein 0401 agriculture forestry and fisheries General Agricultural and Biological Sciences Genomic structure |
| Zdroj: | PeerJ, Vol 8, p e9655 (2020) |
| ISSN: | 2167-8359 |
| Popis: | Myostatin (MSTN) is a negative regulator of muscle growth, which restrains the proliferation and differentiation of myoblasts. To understand the role of twomstngenes ofTakifugu bimaculatus, the full-length cDNAs of 1131 bpTbmstn1and 1,080 bpTbmstn2were obtained from theT. bimaculatus’ genomic database, which encodes 376 and 359 amino acids, respectively. The results of qRT-PCR showed thatTbmstn1was expressed in the eye, kidney, spleen, skeletal muscle, gill, and brain, and the expression level in the skeletal muscle was extremely significantly higher than in other examined tissues.Tbmstn2was expressed in the skin, skeletal muscle, gill, and brain, and had the highest expression in the skeletal muscle, followed by expression in the brain. Meanwhile, in different stages of embryonic development, the expression ofTbmstn1started from the gastrula stage. Its expression in the eye-pigment formation stage and hatching stage was significantly higher than that in other stages. TheTbmstn2was expressed in all examined embryonic stages with different levels, and the highest expression was detected in the eye-pigment formation stage. These results suggested thatTbmstn1andTbmstn2may involve in the development of skeletal muscle, andTbmstn2may be related to the formation of nervous system. |
| Databáze: | OpenAIRE |
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