Multiple-locus variable number of tandem repeats (VNTR) fingerprinting (MLVF) and antibacterial resistance profiles of extended spectrum beta lactamase (ESBL) producing Pseudomonas aeruginosa among burnt patients in Tehran
Autor: | Morovat Taherikalani, Marzieh Aligholi, Babak Khoramian, Fereshteh Jabalameli, Akbar Mirsalehian, Nazli Sotoudeh, Leila Jabalameli, Mohammad Emaneini |
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Rok vydání: | 2011 |
Předmět: |
Imipenem
medicine.medical_treatment Ceftazidime Minisatellite Repeats Aztreonam Iran Critical Care and Intensive Care Medicine Cefpodoxime Polymerase Chain Reaction beta-Lactamases Microbiology chemistry.chemical_compound Drug Resistance Bacterial polycyclic compounds medicine Humans Pseudomonas Infections business.industry General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses DNA Fingerprinting Anti-Bacterial Agents Variable number tandem repeat Phenotype chemistry Amikacin Ticarcillin Pseudomonas aeruginosa Wound Infection Emergency Medicine Beta-lactamase bacteria Surgery Burns business medicine.drug |
Zdroj: | Burns. 37:1202-1207 |
ISSN: | 0305-4179 |
Popis: | Extended spectrum β-lactamase (ESBL)-producing trait was present in 48 out of the 112 (42.8%) Pseudomonas aeruginosa isolates collected from burn wound infections during a 12-month period. The presence of oxa-10, per-1, veb-1 and ges genes and the multiple-locus variable number of tandem repeats (VNTR) fingerprinting (MLVF) of 112 P. aeruginosa strains were determined by PCR and multiplex PCR. Disk diffusion methods were used to determine the susceptibility of the isolates to antimicrobial agents as instructed by CLSI. All ESBL isolates were resistant to aztreonam, cefepime, cefotaxime, cefpodoxime, ceftazidime, ceftriaxone and ofloxacin. Fewer than 60% of ESBL isolates were resistant to imipenem, meropenem, and piperacillin-tazobactam but more than 90% were resistant to amikacin, ciprofloxacin, levofloxacin, ticarcillin and tobramycin. The most prevalent ESBL genes included oxa-10 (70%) and per-1 (50%) followed by veb-1 (31.3%). The gene encodes GES enzyme did not detect in any isolates. A total of 100 P. aeruginosa strains were typed by MLVF typing method. MLVF produced 42 different DNA banding patterns. These data indicate that different MLVF types infect burn wounds in patients at a hospital in Tehran and also suggest an alarming rate of ESBL-producing isolates in this test location. |
Databáze: | OpenAIRE |
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