Molecular typing and genomic characteristic of human adenoviruses in Datong, Northern China
| Autor: | Shahzad Akbar Khan, X.L. Ye, Ying Liu, Xuehua Zheng, Yaqing Li, Liqiang Feng, Xingchen Wang, Zhen Lin, Ling Chen, Qiang Zhou, Bo Chen, Tianxing Ji |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Human Adenoviruses
Mutation virus diseases Biology medicine.disease_cause Virology eye diseases 03 medical and health sciences Molecular typing 0302 clinical medicine Infectious Diseases Molecular evolution Genotype medicine 030211 gastroenterology & hepatology 030212 general & internal medicine Gene Virus classification |
| Zdroj: | Journal of Medical Virology. 92:3111-3118 |
| ISSN: | 1096-9071 0146-6615 |
| DOI: | 10.1002/jmv.26203 |
| Popis: | The molecular prevalence of human adenoviruses (HAdVs) in Datong city and molecular evolution of HAdV-C species is still obscure. Here, we explored the molecular prevalence of HAdVs by simultaneous sequencing of hexon and fiber. Then, the penton gene fragments of HAdV-C species were determined by sequencing. Finally, genomic and proteotyping analysis were performed for exploration of molecular evolution of unique HAdV-6. Our results showed that dominant molecular types of HAdVs were HAdV-3, HAdV-2, and HAdV-1 based on the hexon and fiber genotype. Among H2F2 isolates, P1H2F2 was most common, followed by P2H2F2 and HAdV-89. The clinical symptoms of HAdV-1 or HAdV-2 infected patients were more severe than HAdV-3 infected patients, the prognosis of HAdV-1, HAdV-2, and HAdV-3 infected patients was indifference. Genomic and proteotyping analysis demonstrated that DT15 was different from HAdV-6 prototype, with high-discrepant sequences localized in the E3 region. In conclusion, HAdV-1 and HAdV-2 have a high affinity to infect younger children and cause more severe symptoms than HAdV-3. The E3 gene of HAdV-C species was considered as highly recombination and mutation region. |
| Databáze: | OpenAIRE |
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