Isolation and Purification of Colicin ECL 12, a Bacteriocin That Inhibits Strains of Escherichia coli O157:H7
| Autor: | Dennis G. Olson, Wanda J. Lyon |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
inorganic chemicals
Gel electrophoresis biology Proteolytic enzymes biochemical phenomena metabolism and nutrition biology.organism_classification medicine.disease_cause Microbiology Enterobacteriaceae Biochemistry Bacteriocin Colicin medicine bacteria lipids (amino acids peptides and proteins) Escherichia coli Bacteria Food Science Antibacterial agent |
| Zdroj: | Journal of food protection. 60(12) |
| ISSN: | 1944-9097 |
| Popis: | A swine fecal isolate, identified as Escherichia coli ECL12, was found to produce an antimicrobial substance designated as colicin ECL12. Colicin ECL12 was inhibitory against 20 strains of E. coli O157:H7 previously isolated from both human and bovine feces. Identification of the producer strain was determined phenotypically by biochemical and morphological tests. Colicin ECL12 was sensitive to several proteolytic enzymes. Adsorption of colicin ECL12 to sensitive cells of E. coli O157:H7 was bactericidal, resulting in a 2 log reduction in viable cell counts. Colicin ECL12 was purified from strain ECL12 by cell extraction and ion-exchange chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of colicin ECL12 resolved a single protein with a molecular weight of approximately 65,000. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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