Osteoblastic differentiation potential of human amniotic fluid-derived mesenchymal stem cells in different culture conditions
| Autor: | Suteera Narakornsak, Waleephan Tancharoen, Nathaporn Pangjaidee, Lamaiporn Peerapapong, Sirinda Aungsuchawan, Tanongsak Laowanitwattana, Peeraphan Pothacharoen, Runchana Markmee, Witoon Tasuya, Nonglak Boonma, Junjira Keawdee |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Histology Amniotic fluid Osteocalcin Cell Culture Techniques CD34 Fluorescent Antibody Technique Real-Time Polymerase Chain Reaction 03 medical and health sciences Prenatal Diagnosis medicine Humans CD90 Cells Cultured Osteoblasts Staining and Labeling Chemistry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Osteoblast Amniotic stem cells Cell Biology General Medicine Alkaline Phosphatase Amniotic Fluid Flow Cytometry Cell biology Endothelial stem cell 030104 developmental biology medicine.anatomical_structure Female Stem cell |
| Zdroj: | Acta Histochemica. 120:701-712 |
| ISSN: | 0065-1281 |
| DOI: | 10.1016/j.acthis.2018.07.006 |
| Popis: | Osteoporosis is a bone degenerative disease characterized by a decrease in bone strength and an alteration in the osseous micro-architecture causing an increase in the risk of fractures. These diseases usually happen in post-menopausal women and elderly men. The most common treatment involves anti-resorptive agent drugs. However, the inhibition of bone resorption alone is not adequate for recovery in patients at the severe stage of osteoporosis who already have a fracture. Therefore, the combination of utilizing osteoblast micro mimetic scaffold in cultivation with the stimulation of osteoblastic differentiations to regain bone formation is a treatment strategy of considerable interest. The aims of this current study are to investigate the osteoblastic differentiation potential of mesenchymal stem cells derived from human amniotic fluid and to compare the monolayer culture and scaffold culture conditions. The results showed the morphology of cells in human amniotic fluid as f-type, which is a typical cell shape of mesenchymal stem cells. In addition, the proliferation rate of cells in human amniotic fluid reached the highest peak after 14 days of culturing. After which time, the growth rate slowly decreased. Moreover, the positive expression of specific mesenchymal cell surface markers including CD44, CD73, CD90, and also HLA-ABC (MHC class I) were recorded. On the other hand, the negative expressions of the endothelial stem cells markers (CD31), the hematopoietic stem cells markers (CD34, 45), the amniotic stem cells markers (CD117), and also the HLA-DR (MHC class II) were also recorded. The expressions of osteoblastogenic related genes including OCN, COL1A1, and ALP were higher in the osteogenic-induced group when compared to the control group. Interestingly, the osteoblastogenic related gene expressions that occurred under scaffold culture conditions were superior to the monolayer culture conditions. Additionally, higher ALP activity and greater calcium deposition were recorded in the extracellular matrix in the osteogenic-induced group than in the culture in the scaffold group. In summary, the mesenchymal stem cells derived from human amniotic fluid can be induced to be differentiated into osteoblastic-like cells and can promote osteoblastic differentiation using the applied scaffold. |
| Databáze: | OpenAIRE |
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