| Popis: |
PDF file - 94K, (A) 10 5 WM1617 melanoma cells stably expressing sham control and CD133 shRNA were seeded tissue culture plates. Cell numbers in triplicate wells were counted on days 2, 4, and 7. (B) Melanoma cells were suspended in MCDB153/L15 medium (v/v: 4/1) supplemented with 25 mug/ml bovine pituitary extract, 2 ng/ ml EGF, 2 mug/ml insulin, 4% fetal bovine serum, and 0.25% agar and plated in triplicate at 6 � 10 4 cells/well in 6-well plates. After 3 weeks, colonies were counted using an inverted microscope and percentage of colony formation calculated in 10 random chosen fields. Data shown represent average percentage clonogenicity from two different experiments |
