Identifying sources and estimating glandular output of salivary TIMP‐1

Autor:	Lars Holten-Andersen, S Beier Jensen, Ulrik Lademann, Birgitte Nauntofte, Peter Højrup, Jakob Harslund, Nils Brünner, Hans Jørgen Nielsen, Morten Thaysen-Andersen, Allan Bardow, P. Autzen Usher, Hanne Offenberg, Jesper Reibel
Rok vydání:	2008
Předmět:	Male Pathology medicine.medical_specialty Saliva Clinical Biochemistry Saliva secretion Salivary Glands Minor fluids and secretions stomatognathic system Saliva testing Humans Parotid Gland Medicine Whole saliva Body fluid Tissue Inhibitor of Metalloproteinase-1 Chromatography Optimal sampling business.industry General Medicine stomatognathic diseases Potential biomarkers Mastication Female Parotid saliva business
Zdroj:	Holten-Andersen, L, Beier Jensen, S, Bardow, A, Harslund, J, Thaysen-Andersen, M, Lademann, U, Autzen Usher, P, Offenberg, H, Højrup, P, Reibel, J, Nielsen, H J, Brunner, N & Nauntofte, B 2008, ' Identifying sources and estimating glandular output of salivary TIMP-1 ', Scandinavian Journal of Clinical and Laboratory Investigation. Supplement, vol. 68, no. 7, pp. 548-554 . https://doi.org/10.1080/00365510701883180
ISSN:	1502-7686 0036-5513
Popis:	Objective. Tissue inhibitor of metalloproteinases 1 (TIMP-1) has been identified as a potential biomarker in diseases such as cancer, cardiovascular diseases and diabetes. Since TIMP-1 resides in most tissues and bodily fluids, we evaluated the potential of using saliva to obtain reproducible TIMP-1 measurements in a non-invasive manner. Material and methods. Samples of unstimulated and stimulated whole saliva and saliva collected from individual glands were analysed for TIMP-1 content. A TIMP-1 ELISA was validated for use in saliva testing and the most optimal sampling and handling procedures for reproducible measurements identified. Western blotting and MALDI-TOF mass spectrometry were used for confirmatory analyses. Results. The TIMP-1 ELISA was found suitable for saliva measurements. All saliva secretions contained TIMP-1, but in different concentrations ranging from 2.81 ng/mL in submandibular/sublingual saliva to 173.88 ng/mL in parotid saliva. TIMP-1 concentrations were influenced to a varying degree by fluctuations in flow. We found the lowest output in submandibular/sublingual saliva stimulated with 0.5 % citric acid (3.56 ng/min) and highest output in chewing-stimulated whole saliva (267.01 ng/min). Conclusion. This study shows that saliva contains authentic TIMP-1, the concentration of which was found to depend on gland type and salivary flow. Stimulated whole saliva is suggested as a reliable and easily accessible source for TIMP-1 determinations in bodily fluids.
Databáze:	OpenAIRE
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