Insecticidal effects of a novel polyherbal formulation (HF7) against Culex pipiens L. (Diptera: Culicidae)
| Autor: | Fahd A. Al-Mekhlafi, Mohammed S. Al-Khalifa, Nael Abutaha, Mohamed A. Wadaan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Larva
Traditional medicine biology QH301-705.5 Soxhlet extractor Cytotoxicity Botanical pesticide Histopathology Environmental pollution Mosquito control biology.organism_classification Hexane Eugenol chemistry.chemical_compound chemistry Culex pipiens Instar Original Article Biology (General) General Agricultural and Biological Sciences IC50 |
| Zdroj: | Saudi Journal of Biological Sciences Saudi Journal of Biological Sciences, Vol 29, Iss 1, Pp 279-286 (2022) |
| ISSN: | 2213-7106 1319-562X |
| Popis: | Plant secondary metabolites represent the most efficient and convenient method to control and overcome environmental pollution and insecticidal resistance. This study explored the mosquitocidal activity of the combined extract of seven plants, (HF7) extracted using a Soxhlet extractor against Culex pipiens under laboratory conditions. Exposure of the 3rd instars of Cx. pipiens to HF7 hexane extract resulted in LC50:114.5 μg/mL and LC90:117.0 μg/mL values after 24 h. The ovicidal activities of hexane extract against Cx. pipiens eggs were 21.6%, 48.3%, and 71.6% at 187.5, 93.7, and 46.88 μg/mL, respectively. HF7-treated larvae showed the formation of irregular blebbing of epithelial cells toward the lumen and sloughing into the gut lumen. HF7 extract resulted in 100% adulticidal mortality at the concentration of 3.7 mg/test tube after 30 min of exposure. The IC50 of HF7 extract was 97.03 µg/ml against larvae, at which nuclear and morphological changes were observed. The spectroscopy spectrum of HF7 hexane extract disclosed the presence of 57 different secondary metabolites, among which the dominant compound was eugenol (32.3%). HF7 hexane extract could serve as a botanical insecticide for controlling Cx. pipiens and potentially other mosquito species. |
| Databáze: | OpenAIRE |
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