New bioreactor for mechanical stimulation of cultured tendon-like constructs: design and validation
| Autor: | M. Monleón Pradas, Luis Gil-Santos, Jorge Más-Estellés, María C. Araque-Monrós |
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| Rok vydání: | 2020 |
| Předmět: |
Computer science
Bioreactor Biomedical Engineering Stimulation macromolecular substances 030204 cardiovascular system & hematology Collagen Type I Construct Tendons 03 medical and health sciences Mice 0302 clinical medicine Bioreactors medicine Regeneration Animals Humans Tendon Cells Cultured Mechanical stimulation Tissue Engineering Tissue Scaffolds Regeneration (biology) technology industry and agriculture General Medicine equipment and supplies medicine.anatomical_structure FISICA APLICADA MAQUINAS Y MOTORES TERMICOS Surgery Stress Mechanical 030217 neurology & neurosurgery Biomedical engineering |
| Zdroj: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname |
| ISSN: | 1745-2422 |
| Popis: | [EN] Objective: Although several different types of bioreactors are currently available with mechanical stimulation of constructs or prostheses for tendon regeneration, they are in many cases expensive and difficult to operate. This paper proposes a simple bioreactor to mechanically stimulate up to three constructs for tendon and ligament repair, composed of a stainless-steel frame and an electric motor. Methods: The deformation is produced by a cam wheel, whose eccentricity defines the maximum deformation. The test samples, braids of PLA seeded in surface with mouse fibroblasts, are immersed in the culture medium during mechanical stimulation. Results: Its advantages over existing similar bioreactor designs include: easy renewal of the culture medium and an external electric motor to avoid heating and contamination issues. After 14 days of stretching, the culture samples showed enhanced cellular proliferation and cell fiber alignment in addition to higher production of type I collagen. The cells initially seeded on the braid surface migrated to the inside of the braid. Conclusion: Although the results obtained have a poor statistical basis, they do suggest that the bioreactor could be usefully applied to stimulate constructs for tendon and ligament repair. Anyway, further experiments should be conducted in the future. This paper was funded through a researching contract with the Researching Association of the Textil Industries (AITEX, Alcoi, Spain). |
| Databáze: | OpenAIRE |
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