LncRNA SNHG1 regulates immune escape of renal cell carcinoma by targeting miR‐129‐3p to activate STAT3 and PD‐L1
| Autor: | Jing Li, Jun-Kai Ren, Jin-Xing Wei, Pei Tian, Jin-Jian Yang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Cell Mice Nude CD8-Positive T-Lymphocytes urologic and male genital diseases Mice 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Line Tumor PD-L1 medicine Animals Humans STAT3 Carcinoma Renal Cell Mice Inbred BALB C Gene knockdown biology RNA Cell Biology General Medicine Kidney Neoplasms Gene Expression Regulation Neoplastic Blot 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Cancer research biology.protein RNA Long Noncoding Tumor Escape CD8 |
| Zdroj: | Cell Biology International. 45:1546-1560 |
| ISSN: | 1095-8355 1065-6995 |
| DOI: | 10.1002/cbin.11595 |
| Popis: | Immune escape of renal cell carcinoma (RCC) impacts patient survival. However, the molecular mechanism of long noncoding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) in RCC immune escape remains unclear. Quantitative real-time PCR and western blotting results revealed that the expression of lncRNA SNHG1 and STAT3 were upregulated in RCC tissues and cells and that the expression of miR-129-3p was downregulated. Enzyme-linked immunosorbent assay results revealed the increased levels of immune-related factors (interferon-γ, tumour necrosis factor α, and interleukin-2) in RCC tissues. SNHG1 knockdown or miR-129-3p overexpression inhibited the proliferation and invasion of A498 and 786-O cells, while the proliferation and cytotoxicity of CD8+ T cells increased, which promoted the secretion of immune-related factors. STAT3 overexpression decreased the protective effect of miR-129-3p overexpression on RCC cell immune escape. In addition, miR-129-3p knockdown and STAT3 overexpression decreased the protective effect of lncRNA SNHG1 knockdown on RCC cell immune escape. In addition, PD-L1 expression was downregulated after lncRNA SNHG1 knockdown but upregulated after miR-129-3p knockdown and STAT3 overexpression. Dual-luciferase assays showed that lncRNA SNHG1 targets miR-129-3p, and miR-129-3p targets STAT3. RNA pull-down and RNA immunoprecipitation assays verified the regulatory relationship between SNHG1 and STAT3. In vivo, shSNHG1 prolonged the overall survival of RCC tumour model mice and inhibited RCC tumour growth and immune escape but increased CD8+ T cell infiltration in mice. Our findings provide an experimental basis for elucidating the molecular mechanisms of immune escape by RCC and reveal a novel target to treat this disease. |
| Databáze: | OpenAIRE |
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