Performance of the HPV-16 L1 methylation assay and HPV E6/E7 mRNA test for the detection of squamous intraepithelial lesions in cervical cytological samples
| Autor: | Cui Qiu, Ya Li, Paul Okunieff, Lulu Zhang, Yanfang Zhi, Shouhua Rong, Yong Shen, Jiao-Mei Gong, Xiaofu Li |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Uterine Cervical Neoplasms Biology Sensitivity and Specificity High Resolution Melt Disease severity Virology Cytology BDNA test Humans Transition Temperature RNA Messenger Aged Human papillomavirus 16 Messenger RNA RNA Oncogene Proteins Viral Methylation DNA Methylation Middle Aged Molecular biology DNA methylation RNA Viral Capsid Proteins Female Squamous Intraepithelial Lesions of the Cervix |
| Zdroj: | Journal of Virological Methods. 224:35-41 |
| ISSN: | 0166-0934 |
| Popis: | HPV-16 L1 methylation and E6/E7 mRNA have suggested that they had close relationship with cervical neoplastic progression. This study aimed to evaluate the clinical performance of the HPV-16 L1 methylation assay and E6/E7 mRNA test for detecting high-grade cervical lesions (CIN2+). A total of 81 women with liquid-based cytology (LBC) samples, histological results, and positive HPV-DNA test for HPV type 16 only were included in this study. HPV-16 L1 methylation and E6/E7 mRNA levels were measured using methylation-sensitive high resolution melting (MS-HRM) analysis and Quantivirus®HPV E6/E7 RNA 3.0 assay (bDNA), respectively, in the same residue of LBC samples. The current date showed a positive correlation between the HPV-16 L1 methylation and the E6/E7 mRNA levels. The L1 methylation and mRNA levels both increased with disease severity. The mRNA test method showed higher sensitivity and NPV (98.0 and 91.7% vs. 89.8 and 80.8%), while lower specificity and PPV (34.4 and 69.6% vs. 65.6 and 80.0%), than the L1 methylation assay for detecting histology-confirmed CIN2+. When using the detection method of mRNA test combined with L1 methylation assay, we obtained a sensitivity of 89.8% and a specificity of 71.9%. These findings suggest that assessment of HPV-16 L1 methylation testing combined with E6/E7 mRNA testing may be a promising method for the triage of women with HPV type 16 only. |
| Databáze: | OpenAIRE |
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