Molecular analysis of the low-temperature Escherichia coli phage vB_EcoS_NBD2
| Autor: | Rolandas Meškys, Algirdas Kaupinis, Martynas Skapas, Laura Kaliniene, Aurelija Zajančkauskaitė, Eugenijus Šimoliūnas, Monika Vilkaitytė, Lidija Truncaitė |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Subfamily DNA Repair Sequence analysis General Medicine Genome Viral Biology medicine.disease_cause Genome Molecular biology Coliphages Homology (biology) 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Virology DNA Viral medicine Escherichia coli ORFS Gene DNA Phylogeny |
| Zdroj: | Archives of virology. 163(1) |
| ISSN: | 1432-8798 |
| Popis: | A novel low-temperature Escherichia coli phage vB_EcoS_NBD2 was isolated in Lithuania from agricultural soil. With an optimum temperature for plating around 20 °C, vB_EcoS_NBD2 efficiently produced plaques on Escherichia coli NovaBlue (DE3) at a temperature range of 10–30 °C, yet failed to plate at temperatures above 35 °C. Phage vB_EcoS_NBD2 virions have a siphoviral morphology with an isometric head (65 nm in diameter), and a non-contractile flexible tail (170 nm). The 51,802-bp genome of vB_EcoS_NBD2 has a G + C content of 49.8%, and contains 87 probable protein-encoding genes as well as 1 gene for tRNASer. Comparative sequence analysis revealed that 22 vB_EcoS_NBD2 ORFs encode unique proteins that have no reliable identity to database entries. Based on homology to biologically defined proteins and/or proteomics analysis, 36 vB_EcoS_NBD2 ORFs were given a putative functional annotation, including 20 genes coding for morphogenesis-related proteins and 13 associated with DNA metabolism. Phylogenetic analysis revealed that vB_EcoS_NBD2 belongs to the subfamily Tunavirinae, but cannot be assigned to any genus currently recognized by ICTV. |
| Databáze: | OpenAIRE |
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