Functional Consequences of Interactions between Human Neutrophils and ATP, ATP?S, and Adenosine

Autor: Blair A.M. Walker, Brian E. Hagenlocker, Peter A. Ward
Rok vydání: 1990
Předmět:
Zdroj: Annals of the New York Academy of Sciences. 603:108-118
ISSN: 1749-6632
0077-8923
DOI: 10.1111/j.1749-6632.1990.tb37665.x
Popis: There is an increasing body of evidence suggesting that nucleotides such as ATP can serve as important extracellular factors to bring about significant functional changes in phagocytic cells. For instance, ATP and its analogues (for example, adenosine-5‘-0-( 3-thiotriphosphate), or ATPyS) can interact with neutrophils to cause increases in intracellular calcium.14 This appears to be associated with “priming” of these cells for enhanced superoxide (02 ) responses to agonists such as chemotactic peptides.1.2.5.6 Because platelets contain and, when activated, release substantial amounts of ATP and ADP, mammalian systems have the potential for delivering significant amounts of adenine nucleotides to sites of inflammation. It may well be that in vivo secretion products of platelets will bring about an amplification of oxygen radical formation by phagocytic cells, and so intensify tissue injury at the inflammatory site. This concept is supported both by in vitro and in vivo observationsP ’ In contrast to ATP with respect to phagocytic cells, adenosine has an opposing effect: suppression of 0, responses to chemotactic peptides.’,” In this study, we have assessed the effects of ATP (and/or ATPyS) and adenosine on chemotactic peptide receptor numbers and affinities, the cell membrane content of CR3 (complement receptor 111, or, as defined by monoclonal antibodies, Mol antigen (CDl lb)), and the intracellular changes in calcium induced by exposure of neutrophils to chemotactic peptide.
Databáze: OpenAIRE
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