Transformation of Aspergillus oryzae using the A. niger pyrG gene
| Autor: | Shiela E. Unkles, Jim R. Kinghorn, Peter H. Pouwels, Cees A. M. J. J. van den Hondel, I. E. Mattern |
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| Rok vydání: | 1987 |
| Předmět: |
Genetics
biology Carboxy-Lyases Aspergillus oryzae Genetic Vectors Orotidine-5'-Phosphate Decarboxylase Genetic transfer Aspergillus niger biology.organism_classification Neurospora crassa Transformation (genetics) Aspergillus Transformation Genetic Cotransformation Selection Genetic Molecular Biology Gene Southern blot |
| Zdroj: | Molecular and General Genetics MGG. 210:460-461 |
| ISSN: | 1432-1874 0026-8925 |
| Popis: | A transformation system for Aspergillus oryzae based on the orotidine-5'-phosphate decarboxylase gene (pyrG) was developed. Transformation frequencies of up to 16 transformants per microgram of DNA were obtained with the vector pAB4-1, which carries the pyrG gene of A. niger. Southern blotting analysis showed that vector DNA sequences were integrated into the chromosomal DNA, in various copy numbers and presumably at different sites. Efficient cotransformation of an unselectable gene was also shown. Under the conditions used no transformants were obtained with the equivalent pyr4 gene of Neurospora crassa. |
| Databáze: | OpenAIRE |
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