Analysis of the chronic wound microbiota of 2,963 patients by 16S rDNA pyrosequencing
| Autor: | Lawrence Koenig, Jennifer S. White, Richard A. Wolcott, Randall D. Wolcott, Caleb D. Phillips, Eric Rees, Stephen B. Cox, John D. Hanson |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male 0301 basic medicine Chronic wound medicine.medical_specialty medicine.drug_class Staphylococcus Surgical Wound Antibiotics Dermatology Corynebacterium Biology Staphylococcal infections Varicose Ulcer Microbiology 03 medical and health sciences Pseudomonas RNA Ribosomal 16S Streptococcal Infections Internal medicine medicine Humans Pseudomonas Infections Microbiome Aged Retrospective Studies Aged 80 and over Pressure Ulcer Corynebacterium Infections integumentary system Microbiota Streptococcus Surgical wound Middle Aged Staphylococcal Infections medicine.disease Diabetic foot Diabetic Foot United States 030104 developmental biology Chronic Disease Wounds and Injuries Female Surgery Anaerobic bacteria medicine.symptom Wound healing |
| Zdroj: | Wound Repair and Regeneration. 24:163-174 |
| ISSN: | 1524-475X 1067-1927 |
| Popis: | The extent to which microorganisms impair wound healing is an ongoing controversy in the management of chronic wounds. Because the high diversity and extreme variability of the microbiota between individual chronic wounds lead to inconsistent findings in small cohort studies, evaluation of a large number of chronic wounds using identical sequencing and bioinformatics methods is necessary for clinicians to be able to select appropriate empiric therapies. In this study, we utilized 16S rDNA pyrosequencing to analyze the composition of the bacterial communities present in samples obtained from patients with chronic diabetic foot ulcers (N = 910), venous leg ulcers (N = 916), decubitus ulcers (N = 767), and nonhealing surgical wounds (N = 370). The wound samples contained a high proportion of Staphylococcus and Pseudomonas species in 63 and 25% of all wounds, respectively; however, a high prevalence of anaerobic bacteria and bacteria traditionally considered commensalistic was also observed. Our results suggest that neither patient demographics nor wound type influenced the bacterial composition of the chronic wound microbiome. Collectively, these findings indicate that empiric antibiotic selection need not be based on nor altered for wound type. Furthermore, the results provide a much clearer understanding of chronic wound microbiota in general; clinical application of this new knowledge over time may help in its translation to improved wound healing outcomes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text