Role of human T-cell leukemia virus type 1 X region proteins in immortalization of primary human lymphocytes in culture
| Autor: | Bernhard Fleckenstein, W A Haseltine, Susanne Berchtold, I Radant, Ralph Grassmann, M. Alt, U Ramstedt, Joseph Sodroski |
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| Rok vydání: | 1992 |
| Předmět: |
CD4-Positive T-Lymphocytes
Transcription Genetic Lymphocyte viruses Immunology Molecular Sequence Data Mutagenesis (molecular biology technique) Context (language use) Microbiology Virus Virology medicine Humans Cells Cultured Human T-lymphotropic virus 1 biology Base Sequence virus diseases Gene Products tax biochemical phenomena metabolism and nutrition biology.organism_classification medicine.disease Cell Transformation Viral Gene Products rex Leukemia medicine.anatomical_structure Cell culture Mutagenesis Insect Science Cord blood DNA Viral Research Article |
| Zdroj: | Journal of virology. 66(7) |
| ISSN: | 0022-538X |
| Popis: | Human T-cell leukemia virus type 1 (HTLV-1) immortalizes human CD4+ T lymphocytes in culture. Previous studies show that in the context of a herpesvirus saimiri vector, the sequence of the X region at the 3' end of the HTLV-1 genome is also capable of immortalizing CD4+ lymphocytes in the absence of HTLV-1 structural proteins. The X region of HTLV-1 encodes two trans-acting viral proteins, the 42-kDa Tax protein and the 27-kDa Rex protein. Infection of human cord blood cells with herpesvirus saimiri recombinants which contain HTLV-1 X region sequences defective for expression of tax, rex, or both tax and rex demonstrates that tax function is necessary and sufficient for immortalization of primary human CD4+ cord blood lymphocytes in culture in the context of the herpesvirus saimiri vector. |
| Databáze: | OpenAIRE |
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