Ultrahigh-resolution MRI Reveals Extensive Cortical Demyelination in a Nonhuman Primate Model of Multiple Sclerosis
| Autor: | Jing-Ping Lin, Yeajin Song, Steven Jacobson, Maxime Donadieu, Cecil Chern-Chyi Yen, Pascal Sati, Afonso C. Silva, Daniel S. Reich, Joseph R Guy, Diego Szczupak, Frank Q. Ye, Hadar Kolb, Hannah M. Kelly, Erin S Beck |
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| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Encephalomyelitis Autoimmune Experimental Multiple Sclerosis Cognitive Neuroscience Brain damage 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine biology.animal medicine Animals Humans Child 030304 developmental biology 0303 health sciences medicine.diagnostic_test biology business.industry Multiple sclerosis Experimental autoimmune encephalomyelitis Histological Techniques Marmoset Brain Infant Magnetic resonance imaging medicine.disease Magnetic Resonance Imaging Myelin proteolipid protein Disease Models Animal Brain Injuries Child Preschool Original Article medicine.symptom business 030217 neurology & neurosurgery Preclinical imaging Immunostaining Demyelinating Diseases |
| Zdroj: | Cereb Cortex |
| ISSN: | 1460-2199 |
| Popis: | Cortical lesions are a primary driver of disability in multiple sclerosis (MS). However, noninvasive detection of cortical lesions with in vivo magnetic resonance imaging (MRI) remains challenging. Experimental autoimmune encephalomyelitis (EAE) in the common marmoset is a relevant animal model of MS for investigating the pathophysiological mechanisms leading to brain damage. This study aimed to characterize cortical lesions in marmosets with EAE using ultrahigh-field (7 T) MRI and histological analysis. Tissue preparation was optimized to enable the acquisition of high-spatial resolution (50-μm isotropic) T2*-weighted images. A total of 14 animals were scanned in this study, and 70% of the diseased animals presented at least one cortical lesion on postmortem imaging. Cortical lesions identified on MRI were verified with myelin proteolipid protein immunostaining. An optimized T2*-weighted sequence was developed for in vivo imaging and shown to capture 65% of cortical lesions detected postmortem. Immunostaining confirmed extensive demyelination with preserved neuronal somata in several cortical areas of EAE animals. Overall, this study demonstrates the relevance and feasibility of the marmoset EAE model to study cortical lesions, among the most important yet least understood features of MS. |
| Databáze: | OpenAIRE |
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