Minimizing Post-Infusion Portal Vein Bleeding during Intrahepatic Islet Transplantation in Mice
Autor: | Yuki Cui, Wanxing Cui, Wenyu Gou, Hongjun Wang |
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Rok vydání: | 2021 |
Předmět: |
endocrine system
medicine.medical_specialty endocrine system diseases General Chemical Engineering Islets of Langerhans Transplantation Portal vein Adipose tissue Kidney General Biochemistry Genetics and Molecular Biology Diabetes Mellitus Experimental Islets of Langerhans Mice Diabetes mellitus medicine Animals Distribution (pharmacology) geography geography.geographical_feature_category General Immunology and Microbiology Portal Vein business.industry General Neuroscience medicine.disease Islet Thrombosis Surgery Transplantation medicine.anatomical_structure Liver business |
Zdroj: | Journal of Visualized Experiments. |
ISSN: | 1940-087X |
Popis: | Although the liver is currently accepted as the primary transplantation site for human islets in clinical settings, islets are transplanted under the kidney capsule in most rodent preclinical islet transplantation studies. This model is commonly used because murine intrahepatic islet transplantation is technically challenging, and a high percentage of mice could die from surgical complications, especially bleeding from the injection site post-transplantation. In this study, two procedures that can minimize the incidence of post-infusion portal vein bleeding are demonstrated. The first method applies an absorbable hemostatic gelatin sponge to the injection site, and the second method involves penetrating the islet injection needle through the fat tissue first and then into the portal vein by using the fat tissue as a physical barrier to stop bleeding. Both methods could effectively prevent bleeding-induced mouse death. The whole liver section showing islet distribution and evidence of islet thrombosis post-transplantation, a typical feature for intrahepatic islet transplantation, were presented. These improved protocols refine the intrahepatic islet transplantation procedures and may help laboratories set up the procedure to study islet survival and function in pre-clinical settings. |
Databáze: | OpenAIRE |
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