Dielectrophoresis as a Tool to Reveal the Potential Role of Ion Channels and Early Electrophysiological Changes in Osteoarthritis
| Autor: | Csaba Matta, Rula G. Abdallat, Fatima H. Labeed, Rebecca Lewis, Emily Kruchek |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Membrane potential
dielectrophoresis DEP Chemistry Mechanical Engineering chondrocytes detection ion channels Dielectrophoresis Potassium channel In vitro Article Electrophysiology osteoarthritis Membrane Control and Systems Engineering Gene expression Biophysics TJ1-1570 Mechanical engineering and machinery Electrical and Electronic Engineering Ion channel |
| Zdroj: | Micromachines Micromachines, Vol 12, Iss 949, p 949 (2021) Volume 12 Issue 8 |
| ISSN: | 2072-666X |
| Popis: | Diseases such as osteoarthritis (OA) are commonly characterized at the molecular scale by gene expression and subsequent protein production likewise, the effects of pharmaceutical interventions are typically characterized by the effects of molecular interactions. However, these phenomena are usually preceded by numerous precursor steps, many of which involve significant ion influx or efflux. As a consequence, rapid assessment of cell electrophysiology could play a significant role in unravelling the mechanisms underlying drug interactions and progression of diseases, such as OA. In this study, we used dielectrophoresis (DEP), a technique that allows rapid, label-free determination of the dielectric parameters to assess the role of potassium ions on the dielectric characteristics of chondrocytes, and to investigate the electrophysiological differences between healthy chondrocytes and those from an in vitro arthritic disease model. Our results showed that DEP was able to detect a significant decrease in membrane conductance (6191 ± 738 vs. 8571 ± 1010 S/m2), membrane capacitance (10.3 ± 1.47 vs. 14.5 ± 0.01 mF/m2), and whole cell capacitance (5.4 ± 0.7 vs. 7.5 ± 0.3 pF) following inhibition of potassium channels using 10 mM tetraethyl ammonium, compared to untreated healthy chondrocytes. Moreover, cells from the OA model had a different response to DEP force in comparison to healthy cells this was seen in terms of both a decreased membrane conductivity (782 S/m2 vs. 1139 S/m2) and a higher whole cell capacitance (9.58 ± 3.4 vs. 3.7 ± 1.3 pF). The results show that DEP offers a high throughput method, capable of detecting changes in membrane electrophysiological properties and differences between disease states. |
| Databáze: | OpenAIRE |
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