Evaluation of functionalized mesoporous silica SBA-15 as a carrier system for Ph3Sn(CH2)3OH against the A2780 ovarian carcinoma cell line
| Autor: | Santiago Gómez-Ruiz, Christian Bensing, Marija Mojić, Biljana Dojčinović, Danijela Maksimović-Ivanić, Goran N. Kaluđerović, Sandra Carralero, Sanja Mijatović |
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| Rok vydání: | 2016 |
| Předmět: |
Cell death
Carrier systems Carrier system Mesoporous silica-based materials Cells Caspase 2 Nanotechnology 02 engineering and technology 010402 general chemistry Functionalized 01 natural sciences Inorganic Chemistry Ovarian carcinoma Mode of action Clonogenic assay Clonogenic potential biology Ovarian carcinoma cell Chemistry Organometallics Silica Cellular response Mesoporous silica 021001 nanoscience & nanotechnology 0104 chemical sciences Mesoporous Silica Tin Apoptosis Cell culture biology.protein Biophysics Organotin compound Cytology 0210 nano-technology |
| Zdroj: | Dalton Transactions |
| ISSN: | 1477-9234 1477-9226 |
| Popis: | SBA-15|Sn3, a mesoporous silica-based material (derivative of SBA-15) loaded with an organotin compound Ph3Sn(CH2)3OH (Sn3), possesses improved antitumor potential against the A2780 high-grade serous ovarian carcinoma cell line in comparison to Sn3. It is demonstrated that both the compound and the nanostructured material are internalized by the A2780 cells. A similar mode of action of Sn3 and SBA-15|Sn3 against the A2780 cell line was found. Explicitly, induction of apoptosis, caspase 2, 3, 8 and 9 activation, accumulation of cells in the hypodiploid phase as well as accumulation of ROS were observed. Interestingly, Sn3 loaded in the mesoporous silica-based material needed to reach a concentration 3.5 times lower than the IC50 value of the Sn3 compound, pointing out a higher effect of the SBA-15|Sn3 than Sn3 alone. Clonogenic potential, growth in 3D culture as well as mobility of cells were disturbed in the presence of SBA-15|Sn3. Such behavior could be associated with the suppression of p-38 MAPK. Less profound effect of Sn3 compared to SBA-15|Sn3 could be attributed to a different regulation of p-38 and STAT-3, which are mainly responsible for an appropriate cellular response to diverse stimuli or metastatic properties. |
| Databáze: | OpenAIRE |
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