Identification and characterization of the expression profile of the microRNAs in the Amazon species Colossoma macropomum by next generation sequencing
| Autor: | Fátima Gomes, Márcio Roberto Teixeira Nunes, Sérgio Ricardo Nozawa, Iracilda Sampaio, João L.S.G. Vianez, Horacio Schneider, Jedson Ferreira Cardoso, Luciana Watanabe, Layanna Freitas de Oliveira |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Gene regulatory network An?lise de Sequ?ncia de RNA Computational biology Biology Biologia Computacional DNA sequencing 03 medical and health sciences Sequenciamento de Nucleot?deos em Larga Escala Caraciformes / gen?tica Ecossistema Amaz?nico (BR) MicroRNAs / gen?tica Peixes / anatomia & histologia microRNA Genetics Animals Gene Caraciformes / metabolismo Skin Regulation of gene expression Peixes Amazon rainforest business.industry Sequence Analysis RNA Pele / metabolismo Computational Biology High-Throughput Nucleotide Sequencing Ecossistema Amaz?nico F?gado / metabolismo Biotechnology Transcriptoma MicroRNAs 030104 developmental biology Functional annotation Liver Identification (biology) Characiformes business Transcriptome Express?o G?nica |
| Zdroj: | Repositório Digital do Instituto Evandro Chagas (Patuá) Instituto Evandro Chagas (IEC) instacron:IEC |
| Popis: | Federal University of Par? - Campus of Bragan?a. Institute of Coastal Studies. Laboratory of Genetics and Molecular Biology. Bragan?a, PA, Brazil. Federal University of Par? - Campus of Bragan?a. Institute of Coastal Studies. Laboratory of Genetics and Molecular Biology. Bragan?a, PA, Brazil. Dow AgroSciences. Ribeir?o Preto, SP, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Federal University of Par? - Campus of Bragan?a. Institute of Coastal Studies. Laboratory of Genetics and Molecular Biology. Bragan?a, PA, Brazil. Federal University of Par? - Campus of Bragan?a. Institute of Coastal Studies. Laboratory of Genetics and Molecular Biology. Bragan?a, PA, Brazil. Colossoma macropomum is a resistant species native of Amazonas and Orinoco river basins. It is regarded as the second largest finfish of Solim?es and Amazon rivers, representing a major fishery resource in Amazonas and an important species in tropical aquaculture. MicroRNAs are non-coding endogenous riboregulators of nearly 22 nucleotides that play a key role in post-transcriptional gene regulation of several organisms. We analyzed samples of liver and skin from specimens of C. macropomum using next generation sequencing. The dataset was evaluated using computational programs to check the quality of sequences, identification of miRNAs, as well as to evaluate the expression levels of these microRNAs and interaction of target genes. We identified 279 conserved miRNAs, being 257 from liver and 272 from skin, with several miRNAs shared between tissues, with divergence in the number of reads. The strands miR-5p and miR-3p were observed in 72 miRNAs, some of them presenting a higher number of 3p reads. The functional annotation of the most expressed miRNAs resulted in 27 pathways for the liver and skin mainly related to the ?biological processes? domain. Based on the identified pathways, we visualized a large gene network, suggesting the regulation of selected miRNA over this interactive dataset. We were able to identify and characterize the expression levels of miRNAs in two tissues of great activity in C. macropomum, which stands out as the beginning of several studies that can be carried out to elucidate the influence of miRNAs in this species and their applicability as biotechnological tools. |
| Databáze: | OpenAIRE |
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