Effect of omega-3 polyunsaturated fatty acids on the cytoskeleton: An open-label intervention study

Autor: Simone Schmidt, Jan Philipp Schuchardt, Katharina Möller, Janina Willers, Sabine Riecker, Andreas Hahn
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Male
rac1 GTP-Binding Protein
RHOA
Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften
Biologie
Endocrinology
Diabetes and Metabolism
Clinical Biochemistry
Fluorescent Antibody Technique
Gene Expression
Wiskott-Aldrich Syndrome Protein
Neuronal
Red blood cells
n-3 PUFA
Endocrinology
ROCK1
Gene-expression
Cytoskeleton
cdc42 GTP-Binding Protein
Dewey Decimal Classification::500 | Naturwissenschaften
chemistry.chemical_classification
rho-Associated Kinases
Kinase
Lim Kinases
Cofilin
Cell biology
Biochemistry
ddc:500
Polyunsaturated fatty acid
Adult
Cofilin 1
Down-Regulation
RAC1
macromolecular substances
Biology
Real-Time Polymerase Chain Reaction
Actin-Related Protein 2-3 Complex
F-actin
ddc:570
Fatty Acids
Omega-3
Humans
Actin
Biochemistry
medical
GTPases
Research
Biochemistry (medical)
Wiskott-Aldrich Syndrome Protein Family
chemistry
p21-Activated Kinases
biology.protein
rhoA GTP-Binding Protein
Omega-3 index
Zdroj: Lipids in Health and Disease
Lipids in Health and Disease 14 (2015)
ISSN: 1476-511X
DOI: 10.15488/588
Popis: Background Omega-3 polyunsaturated fatty acids (n-3 PUFAs) show beneficial effects on cardiovascular health and cognitive functions, but the underlying molecular mechanisms are not completely understood. Because of the fact that cytoskeleton dynamics affect almost every cellular process, the regulation of cytoskeletal dynamics could be a new pathway by which n-3 PUFAs exert their effects on cellular level. Methods A 12-week open-label intervention study with 12 healthy men was conducted to determine the effects of 2.7 g/d n-3 PUFA on changes in mRNA expression of cytoskeleton-associated genes by quantitative real-time PCR in whole blood. Furthermore, the actin content in red blood cells was analyzed by immunofluorescence imaging. Results N-3 PUFA supplementation resulted in a significant down-regulation of cytoskeleton-associated genes, in particular three GTPases (RAC1, RHOA, CDC42), three kinases (ROCK1, PAK2, LIMK), two Wiskott-Aldrich syndrome proteins (WASL, WASF2) as well as actin related protein 2/3 complex (ARPC2, ARPC3) and cofilin (CFL1). Variability in F-actin content between subjects was high; reduced actin content was only reduced within group evaluation. Conclusions Reduced cytoskeleton-associated gene expression after n-3 PUFA supplementation suggests that regulation of cytoskeleton dynamics might be an additional way by which n-3 PUFAs exert their cellular effects. Concerning F-actin, this analysis did not reveal unmistakable results impeding a generalized conclusion. Electronic supplementary material The online version of this article (doi:10.1186/1476-511X-14-4) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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