MicroRNA characterization in equine induced pluripotent stem cells
| Autor: | Ximena Garate, Gabriel Neiman, Santiago Gabriel Miriuka, Lucía Natalia Moro, Alejandro La Greca, Ariel Waisman, Guadalupe Amín, Verónica Alejandra Furmento, Carlos Luzzani, Natalia Lucía Santín Velazque, G. Vichera, Gustavo Sevlever |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Nuclear Transfer Techniques Embryology Cellular differentiation Biochemistry pluripotent Animal Cells Medicine and Health Sciences Induced pluripotent stem cell Connective Tissue Cells Mammals Multidisciplinary Stem Cells Eukaryota MicroRNA Cell Differentiation purl.org/becyt/ford/3.1 [https] Cell biology Nucleic acids Medicina Básica Connective Tissue KLF4 Vertebrates Medicine purl.org/becyt/ford/3 [https] Cellular Types Anatomy Stem cell Reprogramming Research Article Pluripotency Homeobox protein NANOG CIENCIAS MÉDICAS Y DE LA SALUD Science Cell Potency Rex1 Neurociencias Induced Pluripotent Stem Cells Equines Biology Kruppel-Like Factor 4 03 medical and health sciences stem cells microRNA Genetics Animals Horses Non-coding RNA Natural antisense transcripts Biology and life sciences Gene Expression Profiling Embryos Organisms Cell Biology Fibroblasts Gene regulation MicroRNAs Biological Tissue 030104 developmental biology Amniotes RNA Gene expression Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 13, Iss 12, p e0207074 (2018) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 1932-6203 |
| Popis: | Cell reprogramming has been well described in mouse and human cells. The expression of specific microRNAs has demonstrated to be essential for pluripotent maintenance and cell differentiation, but not much information is available in domestic species. We aim to generate horse iPSCs, characterize them and evaluate the expression of different microRNAs (miR-302a,b,c,d, miR-205, miR-145, miR-9, miR-96, miR-125b and miR-296). Two equine iPSC lines (L2 and L3) were characterized after the reprogramming of equine fibroblasts with the four human Yamanaka‘s factors (OCT-4/SOX-2/c-MYC/KLF4). The pluripotency of both lines was assessed by phosphatase alkaline activity, expression of OCT-4, NANOG and REX1 by RT-PCR, and by immunofluorescence of OCT-4, SOX-2 and c-MYC. In vitro differentiation to embryo bodies (EBs) showed the capacity of the iPSCs to differentiate into ectodermal, endodermal and mesodermal phenotypes. MicroRNA analyses resulted in higher expression of the miR-302 family, miR-9 and miR-96 in L2 and L3 vs. fibroblasts (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|