Frequent loss of heterozygosity in two distinct regions, 8p23.1 and 8p22, in hepatocellular carcinoma
| Autor: | Chenxi Meng, Hiroshi Hano, Keisuke Nagatsuma, Satoru Chiba, Masahiro Ikegami, Tomoe Lu |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male Carcinoma Hepatocellular Loss of Heterozygosity Biology Neoplasm genetics Loss of heterozygosity chemistry.chemical_compound Gene Frequency Carcinoma medicine Humans Allele frequency Aged Genetics Liver Neoplasms Gastroenterology DNA Neoplasm General Medicine Middle Aged Gene deletion medicine.disease digestive system diseases chemistry Hepatocellular carcinoma Female Rapid Communication Gene Deletion DNA Chromosomes Human Pair 8 Microsatellite Repeats |
| Zdroj: | World Journal of Gastroenterology. 13:1090 |
| ISSN: | 1007-9327 |
| DOI: | 10.3748/wjg.v13.i7.1090 |
| Popis: | To identify the precise location of putative tumor suppressor genes (TSGs) on the short arm of chromosome 8 in patients with hepatocellular carcinoma (HCC).We used 16 microsatellite markers informative in Japanese patients, which were selected from 61 published markers, on 8p, to analyze the frequency of loss of heterozygosity (LOH) in each region in 33 cases (56 lesions) of HCC.The frequency of LOH at 8p23.2-21 with at least one marker was 63% (20/32) in the informative cases. More specifically, the frequency of LOH at 8p23.2, 8p23.1, 8p22, and 8p21 was 6%, 52%, 47%, and 13% in HCC cases. The LOH was significantly more frequent at 8p23.1 and 8p22 than the average (52% vs 22%, P = 0.0008; and 47% vs 22%, P = 0.004, respectively) or others sites, such as 8p23.2 (52% vs 6%, P = 0.003; 47% vs 22%, P = 0.004) and 8p21 (52% vs 13%, P = 0.001; 47% vs 13%, P = 0.005) in liver cancer on the basis of cases. Notably, LOH frequency was significantly higher at D8S277, D8S503, D8S1130, D8S552, D8S254 and D8S258 than at the other sites. However, no allelic loss was detected at any marker on 8p in the lesions of nontumor liver tissues.Deletion of 8p, especially the loss of 8p23.1-22, is an important event in the initiation or promotion of HCC. Our results should be useful in identifying critical genes that might lie at 8p23.1-22. |
| Databáze: | OpenAIRE |
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