Cytokine expression in paraffin wax-embedded tissues from conventional calves
| Autor: | Alejandro Núñez, A.I. Raya, Pedro J. Sánchez-Cordón, M. Pedrera, J.L. Romero-Trevejo, José C. Gómez-Villamandos |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Pathology medicine.medical_specialty Necrosis Cross Reactions Pathology and Forensic Medicine Antigen medicine Mesenteric lymph nodes Animals Humans Paraffin Embedding General Veterinary biology Interleukin Antibodies Monoclonal Immunohistochemistry Lymphatic system medicine.anatomical_structure biology.protein Cytokines Tumor necrosis factor alpha Cattle Female medicine.symptom Antibody |
| Zdroj: | Journal of comparative pathology. 136(4) |
| ISSN: | 0021-9975 |
| Popis: | The cross-reactivity of antibodies against human tumour necrosis factor (TNF)alpha, interleukin (IL)-1alpha, IL-1beta and porcine IL-6, and the distribution of immunolabelled cells were evaluated on paraffin wax-embedded tissues from five healthy calves. The tissues were fixed in 10% buffered formalin or Bouin's solution and processed for structural studies and immunohistochemical studies by the avidin-biotin-peroxidase technique. Bouin's solution proved to be the more suitable fixative and Tween 20 the most effective antigen unmasking technique for increasing detectable antigenicity. Constitutive expression of TNFalpha, IL-1alpha, IL-1beta and IL-6 by different cell populations, mainly macrophage-like cells, was detected. Lymphoid organs displayed a higher presence of immunolabelled cells than did lung, liver or kidney. TNFalpha and IL-1alpha appeared as the predominant cytokines, especially in the gut-associated lymphoid tissue of the ileum and in the regional mesenteric lymph nodes. The results will facilitate investigation of the role of these cytokine-producing cells in inflammatory disease processes in calves. |
| Databáze: | OpenAIRE |
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