DNA from anaerobic adult Ascaris lumbricoides and Hymenolepis diminuta mitochondria isolated by zonal centrifugation
| Autor: | John R. Wells, Austin J. MacInnis, Clint E. Carter |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Male
Mitochondrial DNA Hot Temperature Oviducts Cytosine Nucleotides Genitalia Male Mitochondrion Cell Fractionation Nucleic Acid Denaturation Biochemistry Genetics and Molecular Biology (miscellaneous) chemistry.chemical_compound parasitic diseases Organelle Centrifugation Density Gradient Animals Centrifugation Equilibrium Centrifugation Cell Nucleus biology Ascaris Ovary DNA biology.organism_classification Hymenolepis diminuta Centrifugation Zonal Guanine Nucleotides Mitochondria Rats Microscopy Electron Biochemistry chemistry Pronase Cestoda Female Ascaris lumbricoides Ultracentrifugation |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 262:135-144 |
| ISSN: | 0005-2787 |
| DOI: | 10.1016/0005-2787(72)90226-2 |
| Popis: | 1. Reorienting gradient zonal centrifugation was used to isolate large quantities of mitochondria from the parasitic helminths, Ascaris lumbricoides and Hymenolepis diminuta. This technique may be particularly useful in obtaining mitochondria or other organelles present in small quantities from any tissue. 2. Electron micrographs of mitochondrial DNA released from osmotically shocked mitochondria and from purified mitochondrial DNA revealed a circular molecule with an average contour length of 4.76 ± 0.03 μ m in H. diminuta and 4.79 ± 0.03 μ m in A. lumbricoides. 3. Mitochondrial DNA was subsequently isolated from both helminths and characterized using equilibrium centrifugation in CsCl. The buoyant density of mitochondrial DNA was 1.690 g/cm3 in A. lumbricoides and 1.691 g/cm3 in H. diminuta. Estimates made from buoyant density measurements and thermal transition values indicated a base composition of 31 % G + C for A. lumbricoides and 32 % for H. diminuta mitochondrial DNA. |
| Databáze: | OpenAIRE |
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