The photophysics of LOV-based fluorescent proteins — new tools for cell biology
| Autor: | Janko Potzkei, Christian Rupprecht, Thomas Drepper, Ulrich Krauss, Giorgia Casini, Karl-Erich Jaeger, Thomas Gensch, Stephan Endres, Christoph Fahlke, Marcus Wingen |
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| Rok vydání: | 2014 |
| Předmět: |
Models
Molecular Chemistry Molecular Sequence Data Genetic Variation Nanotechnology Fluorescence Fluorescence spectroscopy Protein Structure Tertiary Green fluorescent protein Specific fluorescence Luminescent Proteins Protein structure Spectrophotometry Escherichia coli Biophysics Fluorometry Amino Acid Sequence Molecular oxygen ddc:620 Physical and Theoretical Chemistry Biological imaging Sequence Alignment |
| Zdroj: | Photochemical & photobiological sciences 13(6), 875-83 (2014). doi:10.1039/C3PP50414J |
| ISSN: | 1474-9092 1474-905X |
| DOI: | 10.1039/c3pp50414j |
| Popis: | LOV-based fluorescent proteins (FPs) are an alternative class of fluorescent reporters with unique properties which complement the well-established proteins of the GFP family. One of the most important features of LOV-based FPs is the independence of molecular oxygen for the development of their specific fluorescence. Furthermore, they are characterized by small size and rapid signal development. Over the last few years, a number of different bacterial and plant LOV-based fluorescent proteins such as FbFP, iLOV and miniSOG have been developed and optimized. In this report, we comparatively have characterized the photophysical properties of nine different LOV-based fluorescent proteins including the excitation and emission maxima, the extinction coefficient, the fluorescence quantum yield, the average fluorescence lifetime and the photostability. The unified characterization of the LOV-based FPs provides a useful guide to apply them as in vivo tools for quantitative analyses and biological imaging. |
| Databáze: | OpenAIRE |
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