Interlaboratory ring test of time-resolved fluoroimmunoassays for zeranol and α-zearalenol and comparison with zeranol test kits
| Autor: | G. Alfredsson, A. Iitia, Kevin M. Cooper, D.G. Kennedy, T. Lovgren, S. Tsitsamis, Saskia S. Sterk, M. Tuomola, Marco Blokland, Luís Ribeiro, P. Alves, Harri Takalo, V. Vozikis |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Stereochemistry
Health Toxicology and Mutagenesis Falso positivo Fluoroimmunoassay Urine Cross Reactions Toxicology chemistry.chemical_compound Fusarium medicine Zeranol Animals media_common.cataloged_instance False Positive Reactions Estrogens Non-Steroidal European union Analysis method media_common Chromatography medicine.diagnostic_test Chemistry Public Health Environmental and Occupational Health Cross reactions Alpha-zearalenol General Chemistry Mycotoxins Substance Abuse Detection Chemistry (miscellaneous) Immunoassay Cattle Reagent Kits Diagnostic Food Science |
| Zdroj: | Food Additives and Contaminants. 20:804-812 |
| ISSN: | 0265-203X |
| Popis: | Many zeranol immunoassay test kits cross-react with toxins formed by naturally occurring Fusarium spp. fungi, leading to false-positive screening results. This paper describes the evaluation and application of recently published, dry reagent time-resolved fluoroimmunoassays (TR-FIA) for zeranol and the toxin alpha-zearalenol. A ring test of bovine urine fortified with zeranol and/or alpha-zearalenol in four European Union National Reference Laboratories demonstrated that the TR-FIA tests were accurate and robust. The alpha-zearalenol TR-FIA satisfactorily quantified alpha-zearalenol in urine fortified at 10-30 ng ml(-1). The specificity-enhanced zeranol TR-FIA accurately quantified zeranol in the range 2-5 ng ml(-1) and gave no false-positive results in blank urine, even in the presence of 30 ng ml(-1) alpha-zearalenol. Zeranol TR-FIA specificity was demonstrated further by analysing incurred zeranol-free urine samples containing natural Fusarium spp. toxins. The TR-FIA yielded no false-positive results in the presence of up to 22 ng ml(-1) toxins. The performance of four commercially available zeranol immunoassay test kits was more variable. Three kits produced many false-positive results. One kit produced only one potential false-positive using a protocol that was longer than that of the TR-FIA. These TR-FIAs will be valuable tools to develop inspection criteria to distinguish illegal zeranol abuse from contamination arising from in vivo metabolism of Fusarium spp. toxins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|