Characterization of lymphocyte subpopulations in sheep by rosette formation, adherence to nylon wool and mitogen responsiveness
| Autor: | P.M. Outteridge, R.M. Binns, S.T. Licence |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Erythrocytes
Rosette Formation Surface Immunoglobulin Immunology Population Fluorescent Antibody Technique Receptors Antigen B-Cell chemical and pharmacologic phenomena Receptors Fc Lymphocyte Activation Rosette (botany) Antigen Null cell Animals Lymphocytes Receptor education Immunosorbent Techniques Phytohaemagglutinin education.field_of_study Sheep General Veterinary biology Molecular biology Biochemistry Concanavalin A Antigens Surface biology.protein |
| Zdroj: | Veterinary Immunology and Immunopathology. 2:3-18 |
| ISSN: | 0165-2427 |
| DOI: | 10.1016/0165-2427(81)90034-9 |
| Popis: | Sheep peripheral blood lymphocytes have been studied using a number of surface markers. Thus 16.6 +/- 2.4% (mean +/- S.E.) were surface immunoglobulin positive (sIg+) by direct immunofluorescence, 35.9 +/- 2.1% formed Fc rosettes with bovine red blood cells (RBC) sensitized with rabbit antibody (Fc+) and 28.4 +/- 2.0% formed rosettes with sheep red blood cells (RBC) in the presence of 4% dextran (DS+). The percentage of both Fc+ and DS+ lymphocytes tended to increase with age of the animals. Demonstration of these markers allowed computation of two further subpopulations: null cells lacking sIg and a receptor for sheep RBC, and Fc.null cells lacking a receptor for Fc and sheep RBC. The former population, which contained a proportion of Fc+ lymphocytes comprised 49.8 +/- 3.8% of blood lymphocytes and the latter 38.4 +/- 3.0%. Separation on nylon wool columns, selective rosette enrichment and depletion on density gradients and stimulation with phytomitogens have shown sIg+ and Fc+ lymphocytes to be nylon wool adherent and unresponsive to phytohaemagglutinin (PHA) and Concanavalin A (Con A) and DS+ lymphocytes to be nylon wool non-adherent and responsive to PHA and Con A. The data also indicates a major overlap of the lymphocyte subpopulations bearing sIg and Fc which are apparently B lymphocytes. Moreover these data support the contention that E-rosette formation with sheep RBC in the presence of dextran is a marker for sheep T cells. The data also indicates tha Fc.null cells are T cells, eluting in the non-adherent fraction from nylon wool. It is probable that a proportion of these cells bear a SRBC receptor too weak for present detection methods. |
| Databáze: | OpenAIRE |
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