Oil-in-water nanoemulsions comprising Berberine in olive oil: biological activities, binding mechanisms to human serum albumin or holo-transferrin and QMMD simulations
| Autor: | Majid Darroudi, Jamshid Mehrzad, Jamshidkhan Chamani, Mohammad Reza Saberi, Atena Sharifi-Rad |
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| Rok vydání: | 2020 |
| Předmět: |
Holo transferrin
Binding Sites Berberine Chemistry Transferrin Water Serum Albumin Human General Medicine Iran Human serum albumin Oil in water chemistry.chemical_compound Spectrometry Fluorescence Structural Biology otorhinolaryngologic diseases medicine Humans Thermodynamics Food science Olive Oil Molecular Biology Protein Binding medicine.drug Olive oil |
| Zdroj: | Journal of Biomolecular Structure and Dynamics. 39:1029-1043 |
| ISSN: | 1538-0254 0739-1102 |
| Popis: | Berberine is widely used in traditional Iranian medicine to treat diabetes and inflammatory conditions. This study was aimed at developing a method for the preparation of Berberine nanoparticles (Nano-Ber) in order to improve its aqueous-phase solubility and its complex formation with human serum albumin (HSA) and holo-transferrin (HTF) from the viewpoint of interaction behavior. Nano-Ber was prepared with olive oil as the oil phase, Tween 80 as the surfactant and Span 60 as the co-surfactant. Nano-Ber was obtained with a spherical shape and a mean particle size of 43.7 ± 3.6 nm, with an optimal oil:surfactant:co-surfactant ratio of 1:2:2, w/w/w. The antioxidant activity of Nano-Ber in comparison with Berberine was tested using DPPH and it was found that Nano-Ber had a large antioxidant activity. The cytotoxicity effects of Nano-Ber and Berberine on HepG2 were compared by MTT assay and detected in the treated HepG2 cells at concentrations up to 0.1 mM. The binding constants of HSA-Nano-Ber and HTF-Nano-Ber complexes formation were (2.93 ± 0.02) × 10 |
| Databáze: | OpenAIRE |
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