Lipidomics biomarkers in women with polycystic ovary syndrome (PCOS) using ultra-high performance liquid chromatography–quadrupole time of flight electrospray in a positive ionization mode mass spectrometry

Autor:	Carmen Socaciu, Gabriela Roman, Camelia Larisa Vonica, Anca Farcas, Bogdan Georgescu, Carmen Emanuela Georgescu, Corina Moraru, Andrada Alina Muresan, Ioana Ilie
Rok vydání:	2019
Předmět:	Adult 0301 basic medicine Spectrometry Mass Electrospray Ionization medicine.medical_specialty endocrine system diseases Clinical Biochemistry Mass spectrometry Risk Assessment Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Metabolic Diseases Lipid oxidation Internal medicine Lipidomics medicine Humans Metabolomics Carnitine Chromatography High Pressure Liquid 030219 obstetrics & reproductive medicine business.industry Polycystic ovary syndrome (PCOS) Hypertriglyceridemia General Medicine medicine.disease Polycystic ovary Cross-Sectional Studies 030104 developmental biology Endocrinology chemistry Female business Biomarkers Polycystic Ovary Syndrome medicine.drug
Zdroj:	Scandinavian Journal of Clinical and Laboratory Investigation. 79:437-442
ISSN:	1502-7686 0036-5513
DOI:	10.1080/00365513.2019.1658215
Popis:	Polycystic ovary syndrome (PCOS), characterized by oligo-anovulation and androgen excess is considered a high-risk condition for metabolic disorders. Herein, untargeted metabolomics analysis was applied to women with PCOS, aiming to provide deeper insights into lipidomics biomarkers signature of PCOS, for better diagnosis and management. This was a cross-sectional study in which 15 Caucasian women with PCOS and 15 Caucasian healthy, age-matched women were enrolled. Lipidomics analysis was performed using Ultra-High Performance Liquid Chromatography-Quadrupole Time of Flight Electrospray Mass Spectrometry. Partial Least Squares Discriminant Analysis retrieved the most important discriminative metabolites. Significantly increased levels of triacylglycerol (18:2/18:2/0-18:0) in addition to cholestane-3beta, 5alpha, 6beta-triol (18:0/0:0) and cholestane-5alpha (18:1/0:0) appeared as valuable variables to differentiate subjects with PCOS from controls. Acyl-carnitine 2-hydroxylauroylcarnitine was significantly elevated in PCOS in opposition to decreased phosphocholines metabolites (18:1/18:4, 18:3/18:2), to suggest a metabolic pattern linked to lipid peroxidation. A high fat intake or reduced fat energy consumption during nighttime due to diminished ability to switch to lipid oxidation during fasting time possibly contribute to hypertriglyceridemia found in PCOS. Furthermore, inflammatory mediators including metabolites of the prostaglandin (PG) E2 pathway and oxo-leukotrienes (LT) were increased in patients with PCOS. Potential lipidomics biomarkers were identified that could stratify between women with PCOS and healthy controls. The results show particular alterations in acylglycerols, PGs and LTs and phosphocholines and carnitine metabolites. The lipidomics profiles of PCOS indicate a higher risk of developing metabolic diseases.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::abd8888f30dcf3b1a980c09bbb7dcde5 https://doi.org/10.1080/00365513.2019.1658215 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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