Suppressive role of Viola odorata extract on malignant characters of mammosphere-derived breast cancer stem cells
| Autor: | Kamran Ghaedi, M H Nasr Esfahani, F Seyed Forootan, T Ghafghazi, Saghar Yousefnia, M Tabatabaeian, D Naseri, F Moattar |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cancer Research Apoptosis Breast Neoplasms Chick Embryo 03 medical and health sciences 0302 clinical medicine Breast cancer Viola Cancer stem cell Cell Movement Cell Line Tumor Spheroids Cellular medicine Animals Humans skin and connective tissue diseases Cell Proliferation business.industry Plant Extracts Viola odorata General Medicine medicine.disease 030104 developmental biology Oncology Cell culture SKBR3 030220 oncology & carcinogenesis Cancer cell Cancer research Neoplastic Stem Cells Female Stem cell business |
| Zdroj: | Clinicaltranslational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico. 22(9) |
| ISSN: | 1699-3055 |
| Popis: | Mammospheres are breast cancer stem cells (BCSCs) that could be yielded through culturing cells in non-adherent and non-differentiating condition. With regard to therapy resistance of cancer stem cells (CSCs), it is essential to discover efficient approaches targeting CSCs. Viola odorata extract has been considered as a traditional herbal anti-metastatic drug in several cancer cells. Effect of this drug on BCSCs has not been clearly identified. Current study tries to detect and to compare effect of Viola odorata extract on malignant characterization of breast cancer cell lines and BCSCs. MCF7 and SKBR3 and their derived mammospheres as BCSCs were used and the effect of alcoholic extraction of Viola odorata on apoptosis and malignant characters of MCF7, SKBR3 and their derived BCSCs were analyzed and compared. Viola odorata extract induced cell death in MCF7, SKBR3 and their derived mammospheres through apoptosis without any effects on MCF10A. Also, this extract showed anti-migratory, anti-invasion and anti-colony formation activity in MCF7, SKBR3 and their derived mammospheres which was significantly more in MCF7- and SKBR3-derived mammospheres. Also, this extract decreased size and volume of tumors generated by MCF7, SKBR3 and their derived mammospheres in chicken embryo model. Viola odorata extract exerted anti-cancerous activity on both breast cancer cell lines and their derived BCSCs. Anti-cancerous activity of this extract was significantly more in MCF7-, SKBR3-derived mammospheres in comparison with dedicated cell lines. Data suggest that Viola odorata extract mostly targets cancerous cells, not normal cells with exception in high concentration. It acts in a cell-dependent manner. |
| Databáze: | OpenAIRE |
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