Real-time metabolomics on living microorganisms using ambient electrospray ionization flow-probe
| Autor: | Maria Månsson, Xueting Liu, Lena Gerwick, Robert A. Quinn, Lixin Zhang, Jinshu Fang, Emad K. Alazzeh, Rachel J. Dutton, Mariam S. ElNaggar, Pieter C. Dorrestein, Kirsten Amalie Møller, Jiri Pikula, Yao Peng, Laura M. Sanchez, Cheng-Chih Hsu, Benjamin E. Wolfe, Samantha J. Mascuch, Yi Zeng |
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| Rok vydání: | 2013 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Time Factors biology Bacteria Electrospray ionization Microorganism Streptomyces coelicolor Microbial metabolism Fungi biology.organism_classification Yeast Article Analytical Chemistry chemistry.chemical_compound Metabolomics chemistry Environmental chemistry Yeasts Food science Nutrient agar |
| Zdroj: | Analytical chemistry. 85(15) |
| ISSN: | 1520-6882 |
| Popis: | Microorganisms such as bacteria and fungi produce a variety of specialized metabolites that are invaluable for agriculture, biological research, and drug discovery. However, the screening of microbial metabolic output is usually a time-intensive task. Here, we utilize a liquid microjunction surface sampling probe for electrospray ionization-mass spectrometry to extract and ionize metabolite mixtures directly from living microbial colonies grown on soft nutrient agar in Petri-dishes without any sample pretreatment. To demonstrate the robustness of the method, this technique was applied to observe the metabolic output of more than 30 microorganisms, including yeast, filamentous fungi, pathogens, and marine-derived bacteria, that were collected worldwide. Diverse natural products produced from different microbes, including Streptomyces coelicolor , Bacillus subtilis , and Pseudomonas aeruginosa are further characterized. |
| Databáze: | OpenAIRE |
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