The Duffy antigen/receptor for chemokines (DARC) is expressed in endothelial cells of Duffy negative individuals who lack the erythrocyte receptor
| Autor: | K. Neote, Terence J. Hadley, J. Hesselgesser, Richard Horuk, Stephen C. Peiper, M. J. Conklyn, Zhao-Hai Lu, K. Ogborne, Zi-Xuan Wang, H. J. Showell, A. W. Martin |
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| Rok vydání: | 1995 |
| Předmět: |
Chemokine
Chemokine CXCL1 Recombinant Fusion Proteins Molecular Sequence Data Immunology Protozoan Proteins Gene Expression Antigens Protozoan Receptors Cell Surface Transfection Polymerase Chain Reaction Veins Proinflammatory cytokine Chemokine receptor Antigen Malaria Vivax Tumor Cells Cultured Humans Immunology and Allergy Genetic Predisposition to Disease Amino Acid Sequence Interleukin 8 Growth Substances Receptor Venule Base Sequence Chemotactic Factors biology Erythrocyte Membrane Interleukin-8 Chemotaxis Articles Endocytosis Cell biology Genes biology.protein Intercellular Signaling Peptides and Proteins Endothelium Vascular Carrier Proteins Duffy Blood-Group System Chemokines CXC |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| DOI: | 10.1084/jem.181.4.1311 |
| Popis: | The Duffy antigen/receptor for chemokines (DARC), first identified on erythrocytes, functions not only as a promiscuous chemokine receptor but also as a receptor for the malarial parasite, Plasmodium vivax. The recent finding that DARC is ubiquitously expressed by endothelial cells lining postcapillary venules provides a possible insight into the function of this receptor because this anatomic site is an active interface for leukocyte trafficking. However, the biological significance of DARC is questionable since it has not yet been determined whether individuals lacking the expression of this protein on their erythrocytes (Duffy negative individuals), who are apparently immunologically normal, express the receptor on endothelial cells. However, we report here that DARC is indeed expressed in endothelial cells lining postcapillary venules and splenic sinusoids in individuals who lack the erythrocyte receptor. These findings are based on immunohistochemical, biochemical, and molecular biological analysis of tissues from Duffy negative individuals. We also present data showing that, in contrast to erythrocyte DARC, cells transfected with DARC internalize radiolabeled ligand. We conclude that the DARC may play a critical role in mediating the effects of proinflammatory chemokines on the interactions between leukocyte and endothelial cells since the molecular pathology of the Duffy negative genotype maintains expression on the latter cell type. |
| Databáze: | OpenAIRE |
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