Characterization of terpene synthase genes potentially involved in black fig fly (Silba adipata) interactions with Ficus carica
Autor: | Mwafaq Ibdah, Mosaab Yahyaa, Rika Kedoshim, Anas Kabaha, Bhagwat Nawade, Liora Shaltiel-Harpaz, Tejas C. Bosamia |
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Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine Oviposition Ficus Plant Science Sesquiterpene Parthenocarpy 01 natural sciences Terpene 03 medical and health sciences chemistry.chemical_compound Cadinene Linalool Botany Genetics Animals Pollination Nerolidol Volatile Organic Compounds biology Diptera fungi General Medicine biology.organism_classification Olfactory Perception 030104 developmental biology chemistry Fruit Female Carica Agronomy and Crop Science 010606 plant biology & botany |
Zdroj: | Plant science : an international journal of experimental plant biology. 298 |
ISSN: | 1873-2259 |
Popis: | The black fig fly (Silba adipata) is one of the major pests of figs worldwide. This study investigated the effect of pollination on black fig fly infestation and volatile emission during fruit development of facultative parthenocarpic Ficus carica. The results from in-field oviposition preference of black fig fly, olfactory analysis, and fruit volatile profiles indicate that the black fig fly gave a strong preference to unpollinated figs that showed higher emissions of volatile organic compounds. Terpenes are known to be important compounds determining many insect-plant interactions, so we report a transcriptome-based identification and functional characterization of a terpene synthase (TPS) gene family in F. carica. The protein expression in Escherichia coli of eight terpene synthases (TPSs) revealed that three were monoterpene synthases belonging to the TPS-b clade, with FcTPS6 catalyzing the formation of 1,8-cineole while the other two converted GPP into linalool. Four sesquiterpene synthases from the TPS-a clade catalyze the formation of germacrene D (FcTPS1), E-β-caryophyllene (FcTPS2), cadinene (FcTPS3) and δ-elemene (FcTPS5) while one sesquiterpene synthase FcTPS4 from the TPS-b clade showed nerolidol synthase activity. Most of the enzymatic products closely matched the volatile terpenes emitted from fig fruits and all the genes were expressed during fruit development. This study provides new insights into fig-insect interactions and understanding the molecular mechanisms of terpene biosynthesis and could provide the foundations for sustainable pest management strategies. |
Databáze: | OpenAIRE |
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