The effect of two ribonucleases on the production of Shiga toxin and stx-bearing bacteriophages in Enterohaemorrhagic Escherichia coli
| Autor: | Patricia B Lodato |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Virulence Factors
Mitomycin Science Virulence Viral Plaque Assay medicine.disease_cause Shiga Toxin 1 Shiga Toxin 2 Microbiology Article Shiga Toxin Ribonucleases fluids and secretions STX2 hemic and lymphatic diseases medicine Bacteriophages Gene Prophage Escherichia coli Infections Multidisciplinary biology Toxin Shiga toxin Gene Expression Regulation Bacterial Bacterial pathogenesis biochemical phenomena metabolism and nutrition bacterial infections and mycoses Transcription antitermination Lytic cycle Enterohemorrhagic Escherichia coli biology.protein bacteria Medicine Plasmids |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Enterohaemorrhagic Escherichia coli (EHEC) comprise a group of intestinal pathogens responsible for a range of illnesses, including kidney failure and neurological compromise. EHEC produce critical virulence factors, Shiga toxin (Stx) 1 or 2, and the synthesis of Stx2 is associated with worse disease manifestations. Infected patients only receive supportive treatment because some conventional antibiotics enable toxin production. Shiga toxin 2 genes (stx2) are carried in λ-like bacteriophages (stx2-phages) inserted into the EHEC genome as prophages. Factors that cause DNA damage induce the lytic cycle of stx2-phages, leading to Stx2 production. The phage Q protein is critical for transcription antitermination of stx2 and phage lytic genes. This study reports that deficiency of two endoribonucleases (RNases), E and G, significantly delayed cell lysis and impaired production of both Stx2 and stx2-phages, unlike deficiency of either enzyme alone. Moreover, scarcity of both enzymes reduced the concentrations of Q and stx2 transcripts and slowed cell growth. |
| Databáze: | OpenAIRE |
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