The effect of miR-7 on behavior and global protein expression in glioma cell lines
| Autor: | Feng Luo, Jin‑Liang Yang, Yong Jun Zhou, Lei Dai, Ze Jun Lu, Yuqin Yao, Yan Zhou, Sheng Yong Liu, Shuang Zhang, Hongwei Tian, Hong Xin Deng |
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| Rok vydání: | 2011 |
| Předmět: |
Proteome
Clinical Biochemistry Immunoblotting Down-Regulation Mice Nude Biology medicine.disease_cause Transfection Biochemistry Analytical Chemistry Mice Cell Movement Peptide Initiation Factors Glioma Cell Line Tumor microRNA medicine Animals Humans Electrophoresis Gel Two-Dimensional Neoplasm Metastasis Regulation of gene expression RNA-Binding Proteins Reproducibility of Results Cell migration medicine.disease Gene Expression Regulation Neoplastic MicroRNAs 14-3-3 Proteins Cancer research Ectopic expression Carcinogenesis Cell Migration Assays EIF5A Neoplasm Transplantation |
| Zdroj: | Electrophoresis. 32(24) |
| ISSN: | 1522-2683 |
| Popis: | Malignant glioma is a common cancer of the nervous system. Despite recent research efforts in cancer therapy, the prognosis of patients with malignant glioma has remained dismal. MicroRNAs are noncoding RNAs that inhibit the expression of their targets in a sequence-specific manner, and a few have been shown to act as oncogenes or tumor suppressors. Here, we aimed at exploring the precise biological role of microRNA-7 (miR-7) and the global protein changes in glioma cell lines transiently transfected with miR-7. Transfection of miR-7 into glioma cell lines causes inhibition of cell migration and invasion and suppression of tumorigenesis. Moreover, ectopic expression of miR-7 inhibits lung metastases of glioma in vivo. Among 65 protein spots with differential expression separated by 2-DE, 37 proteins were successfully identified by MS/MS analysis. Of those, the 25 downregulated proteins, which include 14-3-3ζ, eukaryotic translation initiation factor 5A (EIF5A), and annexin A4, may be downstream targets of miR-7, a finding that could elucidate some aspects of the behavior of glioma cells at the protein level. In conclusion, the absence of miR-7 function could cause downstream molecules to switch on or off, resulting in glioma development, invasion, and metastases. MiR-7-based gene treatment may be a novel anti-invasion therapeutic strategy for malignant glioma. |
| Databáze: | OpenAIRE |
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