Adaptation of staphylococcus xylosus to nutrients and osmotic stress in a salted meat model
| Autor: | Régine Talon, Aurore Vermassen, Valérie Laroute, Pierre Micheau, Emilie Dordet-Frisoni, Anne de la Foye, Sabine Leroy |
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| Přispěvatelé: | Unité de Microbiologie (MIC), Institut National de la Recherche Agronomique (INRA), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Université Fédérale Toulouse Midi-Pyrénées, French 'Ministere de l'Education Nationale, de l'Enseignement Superieur et de la Recherche (M.E.N.E.S.R.), ANR-05-PNRA-020 |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Lysis Meat Osmotic shock 030106 microbiology Population lcsh:QR1-502 approche protéomique staphylococcus xylosus Microbiology stress osmotique lcsh:Microbiology 03 medical and health sciences Glutamate homeostasis élément nutritif education Amino acid synthesis Original Research 2. Zero hunger chemistry.chemical_classification education.field_of_study biology Trancriptome Microbiology and Parasitology Staphylococcus xylosus nutrient adaptation biology.organism_classification meat transcriptome osmotic stress Microbiologie et Parasitologie acide aminé 030104 developmental biology Enzyme [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology chemistry Biochemistry Osmoprotectant escherichia coli |
| Zdroj: | Frontiers in Microbiology (7), . (2016) Frontiers in Microbiology, Vol 7 (2016) Frontiers in Microbiology Frontiers in Microbiology, Frontiers Media, 2016, 7, ⟨10.3389/fmicb.2016.00087⟩ |
| ISSN: | 1664-302X |
| Popis: | International audience; Staphylococcus xylosus is commonly used as starter culture for meat fermentation. Its technological properties are mainly characterized in vitro, but the molecular mechanisms for its adaptation to meat remain unknown. A global transcriptomic approach was used to determine these mechanisms. S. xylosus modulated the expression of about 40-50% of the total genes during its growth and survival in the meat model. The expression of many genes involved in DNA machinery and cell division, but also in cell lysis, was up-regulated. Considering that the S. xylosus population remained almost stable between 24 and 72 h of incubation, our results suggest a balance between cell division and cell lysis in the meat model. The expression of many genes encoding enzymes involved in glucose and lactate catabolism was up-regulated and revealed that glucose and lactate were used simultaneously. S. xylosus seemed to adapt to anaerobic conditions as revealed by the overexpression of two regulatory systems and several genes encoding cofactors required for respiration. In parallel, genes encoding transport of peptides and peptidases that could furnish amino acids were up-regulated and thus concomitantly a lot of genes involved in amino acid synthesis were down-regulated. Several genes involved in glutamate homeostasis were up-regulated. Finally, S. xylosus responded to the osmotic stress generated by salt added to the meat model by overexpressing genes involved in transport and synthesis of osmoprotectants, and Na+ and H+ extrusion. |
| Databáze: | OpenAIRE |
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